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Method for establishing rhesus monkey hepatic fibrosis model by intragastric administration method

A technology for liver fibrosis and rhesus monkeys, which is applied in the fields of pharmaceutical formulations, active ingredients of halogenated hydrocarbons, drug delivery, etc., can solve problems such as correspondence and difficult models, and achieve the effects of high efficiency, high success rate, and simple process

Active Publication Date: 2017-12-29
SICHUAN PROVINCIAL PEOPLES HOSPITAL
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0003] At present, the widely used animal models of liver fibrosis include chemical liver fibrosis model, alcoholic liver fibrosis model, immune liver fibrosis model, bile duct obstruction liver fibrosis model and compound factor liver fibrosis model. Humans and other commonly used small animal models are very mature and convenient animal models, but due to the large genetic background and physiological gap between mice and other small animals and humans, it is difficult to directly compare the models constructed by them with human diseases. correspond

Method used

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  • Method for establishing rhesus monkey hepatic fibrosis model by intragastric administration method
  • Method for establishing rhesus monkey hepatic fibrosis model by intragastric administration method

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Experimental program
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Embodiment 1

[0020] Select 10 male rhesus monkeys aged 2-3 years and weighing 5-9kg as model animals. During the modeling process, they were fed with conventional feed for 15 days at first, and the drinking water was normal tap water. After 15 days, the conventional feed was changed to For high-fat feed, the drinking water is 10% alcoholic water solution (dehydrated ethanol mixed with pure water) for drinking water, and then intragastric administration of CCl 4 Solution, twice a week, each dose of 1.0mL / kg, lasted for 20-25 weeks, the modeling was successful, and when it lasted for 30 weeks, it showed severe fibrosis symptoms.

[0021] Wherein, the high-fat feed includes the following components in weight percentage: 16.78% crude protein, 21.35% crude fat, 10% water, 6.8% ash, 3.2% crude fiber, 0.92% calcium, 0.71% phosphorus, and 0.32% cholesterol.

[0022] The 10% alcohol aqueous solution is prepared by mixing absolute ethanol and pure water at a volume ratio of 1:9.

[0023] CCl 4 The...

experiment example

[0027] Experimental example Evaluation method and results of diagnostic model of rhesus monkey liver injury

[0028] 1. Detection of serum biochemical markers

[0029] Serum biochemical markers mainly include alanine aminotransferase (ALT), aspartate aminotransferase (AST), albumin (ALB), bilirubin (BIL), serum total cholesterol (TCH), triglyceride (TG), clotting time (PT and creatinine (Cr).

[0030] After 10 and 20 weeks of modeling, the experimental rhesus monkeys were fasted for 12 hours, and then injected with 0.5 mL of Sutai for intramuscular anesthesia, 5 mL of blood was collected from the vein of the hind limb, centrifuged at 3000 r / min for 15 minutes, the serum was separated, and the content of biochemical markers in the serum was tested , it was found that the content of each marker was significantly different from that of the control group (P<0.05); the control group was fed with conventional feed and drank tap water throughout the process.

[0031] 2. Histopathol...

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Abstract

The invention provides a method for establishing a rhesus monkey hepatic fibrosis model by an intragastric administration method. The method comprises the following steps: selecting two-to-three-year-old male rhesus monkeys with the body weight of 5 to 9 kg as model animals; feeding high-fat feed in a modelling process, enabling the male rhesus monkeys to drink ethyl alcohol aqueous solution with the volume concentration of 9 to 11 percent, and performing intragastric administration on each rhesus monkey to give a CC14 solution in the modelling process, wherein the intragastric administration is performed twice a week and the dosage is 0.5 to 1.5 mL / kg each time, and a model is established successfully after the intragastric administration lasts for 20 to 25 weeks. The method for establishing the rhesus monkey hepatic fibrosis model by the intragastric administration method is simple, safe, reliable, short in modelling time and high in success rate, and can be used for researching an occurrence mechanism of liver diseases.

Description

technical field [0001] The invention belongs to the technical field of animal model construction, and in particular relates to a method for establishing a rhesus monkey liver fibrosis model by intragastric administration. Background technique [0002] Chronic liver disease is a major disease that endangers the health of the population. Hundreds of millions of people in my country carry hepatitis virus. On this basis, factors such as environmental pollution, grain mildew, general-purpose food additives, and alcoholic and non-alcoholic overeating are superimposed on each other, leading to inflammatory reactions, fatty liver, liver fibrosis, Among them, millions of people develop liver cirrhosis and liver cancer every year, causing huge social and family burdens. At present, traditional clinical drug treatment cannot improve the prognosis of end-stage liver disease, and the severe shortage of donor organs worldwide and the increasingly prominent contradiction between supply and...

Claims

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Application Information

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IPC IPC(8): A61K31/02
CPCA61K9/0053A61K31/02
Inventor 黄孝伦冯天航赖春友魏玲玲施静袁可张恬莹
Owner SICHUAN PROVINCIAL PEOPLES HOSPITAL
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