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Microbial agent for restoring water environment, preparation method and method of combining submerged biological bed to restore water environment

A microbial inoculum and water remediation technology, applied in microorganism-based methods, biochemical equipment and methods, biological water/sewage treatment, etc., can solve problems such as unsatisfactory treatment effect, low content, and large treatment volume. , to achieve the unique effect of the formula

Pending Publication Date: 2017-12-29
安徽瑞驰兰德生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, the treatment volume is large, the content is low, and the enrichment of ammonia nitrogen and organic phosphorus due to non-point source pollution has always been a difficulty in the treatment of black and odorous water bodies.
Relying on physical and chemical methods has the problems of large investment, long construction period, unsustainability, and secondary pollution, and the treatment effect is not satisfactory

Method used

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  • Microbial agent for restoring water environment, preparation method and method of combining submerged biological bed to restore water environment
  • Microbial agent for restoring water environment, preparation method and method of combining submerged biological bed to restore water environment
  • Microbial agent for restoring water environment, preparation method and method of combining submerged biological bed to restore water environment

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Experimental program
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preparation example Construction

[0028] The preparation method of the above-mentioned microbial bacterial agent for repairing the water environment is to successively inoculate and cultivate Bacillus pumilus, Pseudomonas putida, Bacillus polymyxa and Aspergillus niger for mass production respectively, and then mix each The mass-production cultured strains were prepared by cross-antagonistic culture.

[0029] Its specific preparation method comprises the following steps:

[0030] Step 1, inoculate Bacillus pumilus (Bacillus pumilus), Pseudomonas putda (Pesudomonasputda), Bacillus polymyxa (Bacillus polymyxa (Prazmowski) Mace) and Aspergillus niger (Aspergillus niger van Tieghem) respectively on the solid medium, Inoculate and cultivate for 36 to 48 hours at a temperature of 28 to 30°C; agar is used as a solid medium.

[0031] Step 2. Inoculate the strains cultivated in step 1 into liquid culture medium respectively, and cultivate them for 36-48 hours at a culture temperature of 28-30° C. and a shaker flask sp...

Embodiment 1

[0041] The implementation site of this example is Shanghai Minhang Youyou Village

[0042] The river is about 1000 meters long, 10-15 meters wide, 1-1.5 meters deep, and 0.4-0.6 meters thick. Due to the natural discharge of a large amount of domestic sewage and industrial sewage, the river water has been seriously polluted for a long time. The river water bloom phenomenon is obvious, showing eutrophication, low transparency, black silt, reaching the standard of mild black odor. Three groups of monitoring sections are set up in this river course as required, and the specific treatment conditions are shown in Table 1 below.

[0043] Table 1 The results of various indicators in the water body of the two groups of Youyou Village at different stages of river management

[0044]

[0045]

Embodiment 2

[0047] The implementation site of this example is Changxing Wei, Fanchang, Wuhu

[0048] The channel is about 3000 meters long, 20-30 meters wide, 0.8-2.2 meters deep, and 0.5-1.0 meters thick. The river course is located in the Economic Development Zone of Fanchang County, and the enterprises in this area are mainly food processing enterprises. Due to the natural discharge of a large amount of industrial sewage and domestic wastewater, the river water has been seriously polluted for a long time. The river water bloom phenomenon is obvious, showing eutrophication, low transparency, and black silt, reaching the severe black and odor standard. At present, water quality maintenance measures mainly rely on physical measures such as dredging, but black and smelly phenomena are repeated, seriously affecting the surrounding living and production activities. According to the requirements, 4 groups of monitoring sections are set up in this river course, and the specific treatment con...

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Abstract

The invention discloses a microbial agent for restoring water environment, a preparation method and a method of combining a submerged biological bed to restore the water environment. The microbial agent comprises, by weight, 20-40% of bacillus pumilus, 20-35% of pseudomonas putida, 15-25% of bacillus polymyxin and 20-35% of aspergillus niger. The above bacteria are respectively subjected to multiple purification and extraction and multi-generation mass production culture and then respectively put in the submerged biological bed and distributed at corresponding positions of a water body, and the bacteria constantly multiply, ferment and release enzyme to continuously degrade ammonia nitrogen and organic phosphide and sulfide. A biological supplementing method is adopted for restoring the water body, so that treatment effects like de-blacking, deodorizing and re-clarifying.

Description

technical field [0001] The invention belongs to the technical field of treatment of black and odorous water bodies, and in particular relates to a microbial agent for repairing a water environment and a preparation method thereof, and a method for repairing a water environment combined with a submerged biological bed. Background technique [0002] At present, there are a large number of black and odorous water bodies of different sizes and uneven distribution in many areas of our country. One of the important reasons for these black and odorous water bodies is the enrichment and excess of ammonia nitrogen, organic phosphorus, and sulfide. However, the treatment volume is large, the content is low, and the enrichment of ammonia nitrogen and organic phosphorus due to non-point source pollution has always been a difficulty in the treatment of black and odorous water bodies. Treatment by physical and chemical methods has the problems of large investment, long construction period...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C12N1/14C02F3/34C12R1/07C12R1/40C12R1/12C12R1/685
CPCC12N1/14C12N1/20C02F3/34C02F2203/006
Inventor 李建斌武先杨芮文东
Owner 安徽瑞驰兰德生物科技有限公司
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