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A preparing method of an antibacterial and immunity-regulating surface-functionalized electrostatic-spun membrane used for bone regeneration

A surface functionalization and immunomodulation technology, applied in the field of antibacterial and immunomodulatory implant materials, can solve problems such as low bioavailability and achieve the effect of solving clinical challenges

Active Publication Date: 2018-01-09
WUXI ZHONGKE GUANGYUAN BIOMATERIALS
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the clinical application of azithromycin is limited by its low water solubility, low bioavailability (37%), and its systemic side effects (such as diarrhea, abdominal pain, and other gastrointestinal symptoms)

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0027] A method for preparing an antibacterial and immunomodulatory surface-functionalized electrospun membrane for bone regeneration, including the preparation of a medical-grade polycaprolactone membrane, the preparation of a medical-grade polycaprolactone membrane containing a calcium phosphate coating, Preparation of medical-grade polycaprolactone film loaded with azithromycin calcium phosphate coating, wherein the preparation of the medical-grade polycaprolactone film of calcium phosphate coating mainly includes three steps of surface activation, biomimetic mineralization and post-treatment

[0028] The preparation of described medical grade polycaprolactone film is as follows:

[0029] Dissolve 5g of polycaprolactone (Mw=80,000) in a mixture of chloroform and dimethylformamide (the volume ratio of chloroform and dimethylformamide is 9 / 1), stir at room temperature for 4 hours, and prepare the concentration As a 25% (w / v) solution. Put the polymer solution into a 5mL syri...

Embodiment 2

[0037] A method for preparing an antibacterial and immunomodulatory surface-functionalized electrospun membrane for bone regeneration, including the preparation of a medical-grade polycaprolactone membrane, the preparation of a medical-grade polycaprolactone membrane containing a calcium phosphate coating, Preparation of medical-grade polycaprolactone film loaded with azithromycin calcium phosphate coating, wherein the preparation of the medical-grade polycaprolactone film of calcium phosphate coating mainly includes three steps of surface activation, biomimetic mineralization and post-treatment

[0038] The preparation of described medical grade polycaprolactone film is as follows:

[0039] Dissolve 10g of polycaprolactone (Mw=150,000) in a mixture of acetone and dimethylformamide (the volume ratio of acetone and dimethylformamide is 9 / 1), stir at room temperature for 5 hours, and prepare the concentration As a 20% (w / v) solution. Put the polymer solution into a 5mL syringe....

Embodiment 3

[0047] A method for preparing an antibacterial and immunomodulatory surface-functionalized electrospun membrane for bone regeneration, including the preparation of a medical-grade polycaprolactone membrane, the preparation of a medical-grade polycaprolactone membrane containing a calcium phosphate coating, Preparation of medical-grade polycaprolactone film loaded with azithromycin calcium phosphate coating, wherein the preparation of the medical-grade polycaprolactone film of calcium phosphate coating mainly includes three steps of surface activation, biomimetic mineralization and post-treatment

[0048] The preparation of described medical grade polycaprolactone film is as follows:

[0049] Dissolve 10g of polycaprolactone (Mw=50,000) in a mixture of acetone and dimethylformamide (the volume ratio of acetone and dimethylformamide is 9 / 1), stir at room temperature for 5 hours, and prepare the concentration As a 20% (w / v) solution. Put the polymer solution into a 5mL syringe. ...

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PUM

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Abstract

The invention relates to the technical field of antibacterial and immunity-regulating implant materials, and particularly relates to a preparing method of an antibacterial and immunity-regulating surface-functionalized electrostatic-spun membrane used for bone regeneration. The method includes preparing a medical-grade polycaprolactone membrane by electrostatic spinning; coating the membrane witha calcium phosphate coating; and immobilizing an antibiotic that is azithromycin into the membrane through a solvent evaporation technique to obtain a medical-grade polycaprolactone membrane loading the azithromycin and having the calcium phosphate coating. The membrane prepared by the method can be used for medicine delivery for local and controlled positions, thus overcoming a clinical problem that is infection during guided bone regeneration, and avoiding limitation on whole-body administration of the azithromycin. The electrostatic spun membrane and the method have a wide application prospect in the field of biomedicine, especially the orthopedics department field.

Description

technical field [0001] The invention relates to the technical field of antibacterial and immunoregulatory implant materials, in particular to a method for preparing an antibacterial and immunoregulatory surface functionalized electrospun membrane for bone regeneration. Background technique [0002] Guided bone regeneration (GBR) is a technique widely used to restore bone defects. The GBR technique involves the surgical application of a barrier membrane that must fulfill several criteria such as biocompatibility, steric maintenance capacity, and not only for the time period of tissue regeneration but also for the entire duration of the wound maturation and remodeling process. Retention of internal barrier properties. In addition to these physical criteria, bioactivity is considered a desirable feature of GBR membranes to initiate and enhance bone formation. GBR technology is a surgical operation that uses a barrier membrane to provide space maintenance and selective repopul...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61L31/06A61L31/08A61L31/16D04H1/728
Inventor 黄建祥许杉杉孟庆怡
Owner WUXI ZHONGKE GUANGYUAN BIOMATERIALS