A rapid visual detection method for glucose and its preparation and application

Abstract

The invention discloses a rapid visual detection method, preparation and application of glucose. The rapid visual detection method comprises the following reaction steps: (1) oxidizing glucose in a sample by using glucose oxidase (GOD) to generate H2O2; (2) under the catalytic actions of ultraviolet irradiation (hereinafter referred to as hv) and ethylene diamine tetraacetic acid (hereinafter referred to as EDTA), enabling 3,3',5',5-tetramethyl benzidine (hereinafter referred to as TMB) to react with the generated H2O2; (3) judging the concentration of the glucose in the sample through solution absorbance change. Through regulating the photo-catalysis, the glucose to-be-tested sample can be subjected to visual classification and quantitative analysis within a short period of time.

Description

technical field [0001] The invention relates to the field of trace detection, in particular to a fast visual detection method for glucose and its preparation and application. Background technique [0002] Glucose (Glucose, chemical formula C 6 h 12 o 6 ), is the basic element of the human body and the most basic medical raw material, and has a wide range of functions and uses. It can be used in the fermentation industry. As a carbon source for microorganisms, it is the main material of fermentation media, such as antibiotics, monosodium glutamate, vitamins, amino acids, organic acids, enzyme preparations, etc., which require a large amount of glucose, and can also be used for microbial fermentation. Raw material for polysaccharides and organic solvents. With the improvement of people's living standards, glucose is also used as a substitute for sucrose in the food industry, such as candy industry, baking industry, high-end vegetable preservation, beverages and meat produc...

AI Technical Summary

Problems solved by technology

Among them, the titration method is simple in principle, but its sensitivity is not high, and it cannot detect trace samples; the electrochemical analysis method is easy to operate, simple in instrument structure, easy to carry and high in accuracy, but its selectivity is poor, and its detection is easily interfered by other substances, so its practicability remains to be seen. Further improvement; high performance liquid chromatography results are more accurate, high sensitivity, but the sample pretreatment time is long, not suitable for rapid determination, and the equipment is expensive, and there are certain difficulties in promotion and popularization; gas chromatography has a good linear relationship , but glucose needs to be treated with silicon etherification, and the operation is more complicated

Therefore, the current traditional detection methods have the disadvantages of long time, poor sensitivity, low specificity, high false positive rate, and low repetition rate of test results.

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