Nucleic acid, kit and method for simultaneous detection of rot pathogens, yellow phytoplasmas and cluster pathogens

Abstract

The invention relates to a group of nucleic acids, kit and detection method for simultaneously detecting three pathogens such as valsa ceratosperma, yellows phytoplasma and rosette pathogenic bacteria. The nucleic acids used for carrying out multiple PCR (Polymerase Chain Reaction) and simultaneously detecting three pathogens comprise forward and reverse primers of the valsa ceratosperma, yellowsphytoplasma and rosette pathogenic bacteria. The nucleic acids used for carrying out fluorescent quantitative PCR and simultaneously detecting the three pathogens further comprise probes correspondingto the various pathogens. A high-efficiency and sensitive detection method capable of simultaneously detecting the three pathogens such as the valsa ceratosperma, yellows phytoplasma and rosette pathogenic bacteria is established. The method can effectively improve the detection sensitivity and avoid false negative results and can provide a technical means for effectively preventing yellows, canker and leaf spot disease.

Description

technical field [0001] The invention belongs to biological detection technology, and in particular relates to a nucleic acid, a kit and a method for simultaneously detecting rot pathogens, yellowing phytoplasma and cluster pathogens. Background technique [0002] Rot disease, also known as decay (decay), also known as rotten skin disease, stinky skin disease, etc., is a common disease that fruit trees are very prone to, and occurs all over the world. The rot disease mainly harms the branches and trunks of the fruiting trees, and it is difficult to detect in the early stage of the disease, so it is necessary to take preventive measures. In the early stage of the disease, it is not easy to identify from the outside. If you lift the skin of the branches, you can see dark brown to reddish-brown wet spots or yellowish-brown dry spots. Sometimes, the internal lesion area is already large, but it is still difficult to identify from the outside. . When the injury is severe, the co...

AI Technical Summary

Problems solved by technology

At present, there is still a lack of high-efficiency fungicides and disease-resistant varieties for apple yellowing, rot and cluster diseases. Once infected with these four diseases, it is necessary to remove the diseased trees in time to prevent the spread of the disease. Therefore, it is particularly important to establish an early, rapid and simultaneous detection method for the detection of pathogenic bacteria that cause apple rot, yellowing and cluster diseases

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