Method of simultaneously detecting residues of different types of veterinary drugs in fish meat

Abstract

The invention discloses a method of simultaneously detecting residues of different types of veterinary drugs in fish meat. In the method, a sample is subjected to pretreatment with a mixture solutioncomposed of acetonitrile, methanol and water according to the volume ratio of 3:1:1 and contains 1% of acetic acid (v / v) and 10 mM of EDTA disodium. A purification operation is composed of one time oflow temperature standing treatment and two times of low-temperature high-speed centrifugation, so that majority of co-extracted protein and lipid impurities are removed. Finally, qualitative detection and quantitative analysis to all target substances are completed by means of tandem of ultra-high performance liquid chromatography and Orbitrap high-resolution mass spectrum under positive and negative ionization modes. The method can simultaneously detecting eighty kinds, covering twelve types, of common fish-use veterinary drug compounds, thus achieving general detection of the veterinary drug compounds in fish meat at high sensitivity, broad spectrum, high speed and high throughput. The method includes simple and high-effective pre-treatment process, so that extraction efficiency of theresidual compounds in various types is ensured. Tandem ultra-high performance liquid chromatography and Orbitrap high-resolution mass spectrum represents high selectivity and sensitivity, so that screening and quantification to potential target substances in the sample can be completed at a lower level.

Description

technical field [0001] The invention relates to a method for detecting drug residues in food, in particular to a method for simultaneously detecting 80 kinds of veterinary drug residues in 12 categories in fish meat based on high-resolution mass spectrometry, and belongs to the technical field of drug residue detection. Background technique [0002] As one of the important areas of national economic development, aquaculture has been greatly developed in the world in recent decades. There is no doubt that veterinary medicine has played a very important role in this. The report on the state of world fisheries and aquaculture released by the Food and Agriculture Organization of the United Nations (FAO) in 2016 pointed out that in 2014, the world's fish consumption had reached a new record of 20kg per person per year, and the supply of fish farming to consumers also increased. For the first time, wild catch has been surpassed, and the trend will continue. However, fish farming...

AI Technical Summary

Problems solved by technology

However, the process of this method is complicated, and it needs to consume a large amount of organic reagents, and requires related extraction devices and consumables, and it is easy to cause a certain degree of loss of the target stream during the column elution process.

In addition, there are also some reports that study the processing method of dispersive solid phase microextraction (dSPE) in order to reduce costs and improve efficiency, but some additional reagent consumables, nitrogen blowing, reconstitution and other tedious operations are still required.

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