Genome IIB type restriction endonuclease site prediction method and electronic equipment
A prediction method and technology of electronic equipment, applied in gene-related fields, can solve the problems of wasting time, cost and funds, and achieve the effect of avoiding time costs and funds
Active Publication Date: 2018-01-19
INST OF ZOOLOGY GUANGDONG ACAD OF SCI
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[0005] However, in the existing technology for cutting genomic DNA with type IIB restriction endonucleases, only relying on pre-experiments to cut DNA with type IIB restriction endonucleases to realize the statistics of enzyme cutting sites, resulting in a waste of too much time and cost and funding
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[0042] The present invention will be described in further detail below in conjunction with the accompanying drawings and specific embodiments.
[0043] Such as figure 1 Shown is a work flow chart of a genome type IIB restriction endonuclease site prediction method of the present invention, including:
[0044] Step S101, obtaining the type IIB restriction endonuclease to be predicted and the genome information to be predicted;
[0045] Step S102, performing restriction site prediction on the genome to be predicted based on all type IIB restriction endonucleases to be predicted, to obtain all restriction site information;
[0046] Step S103, according to the restriction position information, counting and outputting the sequence of restriction endonucleases corresponding to all the type IIB restriction endonucleases to be predicted in the genome to be predicted;
[0047] Step S104, calculating and outputting the interval length between the restriction fragment sequences accordi...
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The invention discloses a genome IIB type restriction endonuclease site prediction method and electronic equipment. The method comprises the steps that information of to-be-predicted IIB type restriction endonucleases and a to-be-predicted genome is acquired; enzyme cutting site prediction is conducted on all the to-be-predicted IIB type restriction endonucleases in the to-be-predicted genome to obtain information of all enzyme cutting positions; enzyme cutting fragment sequences corresponding to all the to-be-predicted IIB type restriction endonucleases in the to-be-predicted genome are counted and output according to the information of the enzyme cutting positions; the interval lengths of the enzyme cutting positions are calculated and output according to the enzyme cutting positions; and the interval lengths are counted. According to the method, cutting of the IIB type restriction endonucleases on DNA is simulated and the enzyme cutting sites are counted through a computer to serveas a reference for a follow-up experiment, and therefore the condition that too much time cost and expenditure are wasted in a pre-experiment is avoided.
Description
technical field [0001] The invention relates to the technical field related to genes, in particular to a genome type IIB restriction endonuclease site prediction method and electronic equipment. Background technique [0002] Type IIB restriction endonucleases are different from traditional restriction endonucleases in that they can recognize specific sequences and cut off flanking sequences at a certain distance from the recognition site ( figure 1 ). Currently, there are 27 type IIB restriction endonucleases reported, 11 of which can be purchased from reagent companies. [0003] Due to the characteristic that type IIB restriction endonucleases can recognize specific DNA sites and cut a fixed length, a method combining type IIB restriction endonucleases with RADseq high-throughput sequencing technology (2b-RAD) has been developed in recent years. . The experimental process first cuts the genomic DNA with a type IIB restriction endonuclease, recovers short sequences of equ...
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IPC IPC(8): G06F19/18G06F19/22G06F19/24
Inventor 陈大嵩戴建青
Owner INST OF ZOOLOGY GUANGDONG ACAD OF SCI