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Molecular marker capable of simultaneously indicating and identifying spinning count and curvature property of sheep wool and application

A molecular marker and curvature technology, which can be used in the determination/inspection of microorganisms, biochemical equipment and methods, DNA/RNA fragments, etc., and can solve the problems of early selection and rapid selection.

Active Publication Date: 2018-01-26
新疆畜牧科学院畜牧研究所 +1
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] The invention provides a molecular marker capable of simultaneously predicting and identifying the traits of sheep wool fineness count and curvature and its specific primer pair, and the specific primer pair can simultaneously predict and identify sheep wool fineness The application of the preparation or kit of molecular markers for the traits of count and tortuosity, the application of the molecular markers in simultaneously predicting and identifying the traits of fineness, count and tortuosity of Chinese Merino sheep wool overcomes the above-mentioned existing Insufficient technology, it can effectively solve the problem of incapable of early and rapid seed selection in the existing process of breeding high-quality sheep breeds with high wool fineness, count and low curvature; Application of SNP loci in the selection of simultaneously predicting and identifying traits of fineness, count and curvature of Chinese Merino sheep wool

Method used

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  • Molecular marker capable of simultaneously indicating and identifying spinning count and curvature property of sheep wool and application
  • Molecular marker capable of simultaneously indicating and identifying spinning count and curvature property of sheep wool and application
  • Molecular marker capable of simultaneously indicating and identifying spinning count and curvature property of sheep wool and application

Examples

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Embodiment 1

[0021] Example 1: A molecular marker capable of simultaneously predicting and identifying sheep wool fineness count and curvature traits, which is located at the 23rd exon of FAT3 gene chromosome 21 (physical location 1501292), the 23rd exon The nucleotide sequence of is shown in the sequence listing SEQ ID No.1.

Embodiment 2

[0022] Example 2: The specific primer pair for amplifying molecular markers that can simultaneously predict and identify sheep wool fineness count and tortuosity traits described in the above examples, including two allele-specific primers (ASP1 / ASP2), a locus-specific primer (LSP) and a specific targeting amplification primer (STA), the sequence of an allele-specific primer (ASP1) is shown in the sequence table SEQ ID No.2, and the other The sequence of the allele-specific primer (ASP2) is shown in the sequence table SEQID No.3, the sequence of the locus-specific primer is shown in the sequence table SEQ ID No.4, and the sequence of the specific targeting amplification primer is shown in the sequence The list is shown in SEQ ID No.5.

Embodiment 3

[0023] Example 3: The application of the specific primer pair described in Example 2 above as a preparation or kit for the preparation of molecular markers for in vitro detection that can simultaneously predict and identify the traits of sheep wool fineness count and tortuosity.

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Abstract

The invention relates to the technical field of animal molecular markers and applications, in particular to a molecular marker capable of simultaneously indicating and identifying the spinning count and the curvature property of sheep wool and an application of molecular marker in simultaneously indicating and identifying the spinning count and the curvature property of the wool of Chinese Merinosheep. The molecular marker capable of simultaneously indicating and identifying the spinning count and the curvature property of sheep wool is located at the 23th exon of No.21 chromosome of the FAT3gene. The invention firstly discloses an rs601824181 SNP site which is taken as the molecular marker capable of simultaneously indicating and identifying the spinning count and the curvature propertyof sheep wool. The rs601824181 SNP site is applied as a selection to simultaneously indicate and identify the spinning count and the curvature property of the wool of Chinese Merino sheep, early selection of varieties of breeding sheep with excellent spinning count and curvature property of sheep wool can be performed, and sheep can be selected and remained after being born.

Description

technical field [0001] The invention relates to the technical field of animal molecular markers and their applications, and is a molecular marker capable of simultaneously predicting and identifying the fineness, count and curvature of sheep wool and its specific primer pair. The specific primer pair is used as a preparation for in vitro detection Application in the preparation or kit of molecular markers for simultaneously predicting and identifying the fineness count and tortuosity traits of sheep wool, the molecular markers are used in simultaneously predicting and identifying the fineness count and tortuosity traits of Chinese Merino sheep wool Applications. Background technique [0002] As the wool spinning industry at home and abroad continues to update the processing of wool and cloth art, the requirements for wool raw materials are more stringent, the product trend has been developing in the direction of lightness and softness, and a new milestone has appeared in the...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/6888C12N15/11
Inventor 田可川黄锡霞赵冰茹田月珍徐新明付雪峰石刚吴伟伟哈尼克孜张艳花于丽娟马桢拉扎提姜徽
Owner 新疆畜牧科学院畜牧研究所
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