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A kind of targeted inhibitor of mtf1 gene and application thereof

An inhibitor and targeted technology, applied in the field of medicine, can solve the problems such as the survival rate of less than 13%, the median survival period of only 12 months, and the blank application of MTF1, so as to achieve no adverse reactions, accurate treatment effect, and reduce Effects of drug resistance problems

Inactive Publication Date: 2020-01-21
SHENGJING HOSPITAL OF CHINA MEDICAL UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, because gliomas have the biological characteristics of invasive growth, and the higher the grade of gliomas, the stronger the invasiveness, even if the surgery achieves total resection under imaging and microscopy, recurrence is still inevitable
The current mainstream surgery plus concurrent radiotherapy after surgery shows that the median survival time is only 12 months, and the 5-year survival rate is less than 13%.
[0005] At present, the role of MTF1 gene in the development of glioma and related mechanisms have not been reported, and the application of MTF1 in glioma gene therapy is still blank.

Method used

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  • A kind of targeted inhibitor of mtf1 gene and application thereof
  • A kind of targeted inhibitor of mtf1 gene and application thereof
  • A kind of targeted inhibitor of mtf1 gene and application thereof

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Experimental program
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Effect test

Embodiment 1

[0039] 1. Culture of U87 and U251 glioma cells.

[0040] (1) Both U87 and U251 cells were cultured in DMEM high-glucose medium containing 10% fetal bovine serum. Observe the cells under an inverted microscope, and passage when the cell confluence is 80%-90%;

[0041] (2) Discard the culture medium in the original bottle, wash 2 times with PBS pre-warmed at room temperature, and discard the PBS;

[0042] (3) Add about 1ml of pre-warmed 0.25% trypsin digestion solution, and observe under a microscope. When the cell protrusions disappear and the cell shape becomes round, discard the digestion solution immediately, and stop the digestion with DMEM high-glucose medium containing 10% fetal bovine serum;

[0043] (4) Use a pipette to pipette the cells into a single-cell suspension, passage at a ratio of 1:3, and place at 37°C, 5% CO 2 Cultivated in a constant temperature incubator;

[0044] (5) The recovered cells can be used for subsequent experiments after 3 passages.

[0045]...

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Abstract

The invention belongs to the technical field of medicines, in particular to a targeted inhibitor for an MTF1 (Metal Regulatory Transcription Factor 1) gene and application thereof. The targeted inhibitor for the MTF1 gene is characterized in that the gene sequence of the targeted inhibitor is 5'-GGATACAAATCACTCACTTTG-3'(SEQ ID No.1). The inhibitor can inhibit the expression of the MTF1 gene in brain glioma cells so as to achieve an effect of treating brain glioma, and has a wide application prospect.

Description

technical field [0001] The invention belongs to the technical field of medicine, and in particular relates to a targeted inhibitor of MTF1 gene and its application. Background technique [0002] Malignant glioma is the most common malignant tumor of the central system, accounting for 50%-60% of primary brain tumors. Currently, the main treatment methods are surgery, with chemotherapy and radiotherapy as adjuvant therapy. However, because gliomas have the biological characteristics of invasive growth, and the higher the grade of gliomas, the stronger the invasiveness, even if the surgery achieves total resection under imaging and microscopy, recurrence is still inevitable. The current mainstream surgery plus concurrent postoperative radiotherapy statistics show that the median survival time is only 12 months, and the 5-year survival rate is less than 13%. The prognosis of high-grade gliomas is even worse. For example, the efficacy of glioblastoma multiforme (GBMs) is still ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N15/113A61K48/00A61K31/7105A61P35/00
Inventor 刘云会薛一雪蔡恒郑健刘啸白阮雪雷李振马珺王振华奚卓刘丽波陈佳佳王萍巩威
Owner SHENGJING HOSPITAL OF CHINA MEDICAL UNIV
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