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A method for constructing tomato pollen viability gradient

A construction method and a technology for pollen vigor, applied in the field of plant cultivation, can solve the problems of decreased pollen germination rate, limited pollen quantity, high requirements for equipment and pollination technology, etc. less demanding effects

Active Publication Date: 2020-09-22
潍坊安普未来生物科技有限公司
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  • Abstract
  • Description
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AI Technical Summary

Problems solved by technology

[0003] The method in the prior art can realize the precise control of the number of active pollen, but the operation is complicated and requires high equipment and pollination technology
At the same time, because the amount of pollen obtained by the stigma is limited, the pollen germination rate decreases

Method used

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  • A method for constructing tomato pollen viability gradient
  • A method for constructing tomato pollen viability gradient

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Embodiment 1

[0022] In the present invention, the unloose powdered stamens are collected one day before tomato flowering, one part is placed in an incubator at 65 degrees Celsius for two hours, and then dried and stored at room temperature; the other part is placed in a drying kettle and dried overnight at room temperature, and can be stored at 4 degrees Celsius after drying. 1 week, should be used as soon as possible.

[0023] The constructed pollen mixture has 7 gradients, the proportions of non-inactivated stamens are 2.5%, 5.6%, 12.5%, 25%, 50%, 100%.

[0024] After mixing different proportions of inactivated stamens and non-inactivated stamens, vortex and shake at 3000 rpm for 3 minutes, so that the dried stamens are fully powdered. Subsequently, use a normal temperature and low-speed centrifuge to centrifuge at 1500 gravitational acceleration for 3 minutes, carefully remove the upper stamen fragments with tweezers, and complete the construction of the tomato pollen activity gradient....

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Abstract

The invention provides a tomato pollen viability gradient construction method. The method includes steps: 1) acquiring non-dispersed stamens one day before tomato flowering, inactivating a part of thestamens in an incubator at 60-70 DEG C, and drying the other part of the stamens overnight in a drying kettle at the room temperature; 2) mixing inactivated stamens and non-inactivated stamens in different proportions, performing vortex oscillation to completely disperse the dried stamens, centrifuging for 2-10min, and removing upper stamen fragments to finish tomato pollen viability gradient construction. By substitution of pollen count with stamen count, a required pollen viability gradient is constructed through matching of the inactivated and non-inactivated stamens in different proportions. The method is simple and convenient to operate, low in requirement on equipment and pollination techniques, and control of stigma activated pollen proportion can be realized through the pollen viability gradient constructed according to the method.

Description

technical field [0001] The invention belongs to the field of plant cultivation, and in particular relates to a method for constructing plant tissues with gradient vitality. Background technique [0002] In the evaluation of tomato pollination effect and research on the promotion of tomato fruit development by pollination, it is necessary to control the number of active pollen. The currently widely used method is to collect active pollen, count the pollen first, and then smear a specific amount of pollen on the tomato stigma. For example, the patent CN 104313107 A discloses a method for identifying the pollen viability of pineapple. The method needs to prepare a culture solution first, place a glass slide in a glass petri dish, then pour the culture solution into and sprinkle with the pineapple that has been preserved for 0-24 hours after harvesting. After the pollen is cultivated for 2-4 hours, the germination of the pollen and the length of the pollen tube are observed und...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N33/00
Inventor 杨慧鹏黄家兴李继莲罗术东吴杰
Owner 潍坊安普未来生物科技有限公司
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