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Estimation Method of Microorganism Quantity Based on Image Processing
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A technology of microbial quantity and image processing, which is applied in computing, computer components, instruments, etc., can solve the problems of experimenter's danger, cumbersome operation, and difficulty in rapid computer realization.
Active Publication Date: 2020-05-12
HARBIN INST OF TECH AT WEIHAI
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This method is suitable for samples with high cell concentration under solid or liquid conditions, but the operation procedure is more complicated
[0008] It can be seen that the existing methods for measuring the number of microorganisms are relatively cumbersome to operate and require the use of biological experiments for measurement, and because there are a considerable number of steps that require manual operations, if the number of harmful bacteria is measured, there is a certain potential danger for the experimenter.
In addition, each method has special requirements for the microorganisms to be measured, and it is difficult to quickly implement it with a computer.
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example 1
[0068] Example 1: In this example, two photos of the same petri dish were taken at different times ( Figure 3-1 , Figure 3-2 ) for bacterial statistics, the specific process is as follows.
[0069] 1: Read the original image I, and perform grayscale processing to obtain the grayscale image I 0 ;
[0070] 2: Remove the part outside the edge of the petri dish in the image: find the radius and center of the contour circle of the petri dish, set the gray level of the edge contour to 0, and set the processed image to I 1 ;
[0071] 3: Use non-local mean filtering algorithm to I 1 Carry out denoising processing, set the image after denoising as I 2 . The specific steps are:
[0072] (1) Suppose the currently processed pixel is i, set Ω i is a search window centered on pixel i with a size of 5×5. Let a certain pixel in the search area be j, N i , N j Represent the neighborhood windows centered on i and j respectively, with a size of 2×2. N i The gray value vector forme...
example 2
[0113] Example 2: In this example, two photos of the same petri dish were taken at different times ( Figure 3-3 , Figure 3-4 ) for bacterial statistics, the specific process is as follows.
[0114] 1: Read the original image I, and perform grayscale processing to obtain the grayscale image I 0 ;
[0115] 2: Remove the part outside the edge of the petri dish in the image: find the radius and center of the contour circle of the petri dish, set the gray level of the edge contour to 0, and set the processed image to I 1 ;
[0116] 3: Use non-local mean filtering algorithm to I 1 Carry out denoising processing, set the image after denoising as I 2 . The specific steps are:
[0117] (1) Suppose the currently processed pixel is i, set Ω i is a search window centered on pixel i with a size of 5×5. Let a certain pixel in the search area be j, N i , N j Represent the neighborhood windows centered on i and j respectively, with a size of 2×2. N i The gray value vector forme...
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Abstract
The present invention belongs to the application in the field of digital imageprocessing. Specifically, certain processing steps are adopted for the image of the bacterial petri dish taken under the microscope, so that the number of pixels in the processed binary image can be used to estimate the number of bacteria in the petri dish. Estimation method of the number of microorganisms based on image processing, based on the binary processing of images, a new method of measuring the number of microorganisms is proposed. This method does not need to conduct experiments on the bacteria themselves, but processes the images of the petri dishes taken under the microscope , belongs to a kind of method for indirectly measuring the number of microorganisms. In the present invention, the non-local mean (NL-means) denoising algorithm is applied, and the statistical results of the bacterial images taken at different times of the same petri dish are relatively stable, and are not affected by changes in bacterial positions. And produce larger error, stronger adaptability.
Description
Technical field: [0001] The present invention belongs to the application in the field of digital image processing. Specifically, certain processing steps are adopted for the image of the bacterial petri dish taken under the microscope, so that the number of pixels in the processed binary image can be used to estimate the number of bacteria in the petri dish. Estimation method of microbial population based on image processing. Background technique: [0002] At present, the commonly used methods for determining the number of microorganisms include microscope direct counting method, plate colony counting method, photoelectric turbidimetric method, cell weight measurement method, and celltotal nitrogen or total carbon content determination. [0003] The microscope direct counting method is to take a certain volume of sample cell suspension and put it in the counting chamber of the counter, and observe and count it with a microscope. It is a fast and intuitive method. The common...
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