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Online toxicity monitoring device and monitoring method of bioelectrochemical system

A bioelectrochemical and monitoring device technology, which is applied in the field of on-line toxicity monitoring devices for bioelectrochemical systems, can solve the problems of expensive proton exchange membranes, easy poisoning of cathode catalysts, and limited pressure tolerance.

Active Publication Date: 2018-02-13
CHANGCHUN INST OF APPLIED CHEMISTRY - CHINESE ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the cathode becomes the limiting factor of the battery, and the test results are easily affected by the performance of the cathode, which greatly hinders its development and commercial application.
HATOX-2000 has the following inherent defects: (1) The cathode chamber requires continuous aeration, which consumes a lot of energy; (2) The cathode catalyst is easily poisoned, and the slow reaction kinetics and catalyst poisoning reduce the cathode potential and the overall efficiency of MFC; (3) Pt reserves are scarce and expensive; (4) The structure and operation are relatively complicated and the maintenance cost is high; (5) Expensive proton exchange membrane is required, and because of the ion exchange membrane between the cathode and the anode, the flow cell, that is, the anode chamber, bears limited pressure
Existing M3C toxicity monitoring reactors can be classified into two types: large-scale reactors and microfluidic reactors. However, the degradation efficiency of large-scale reactors is low, and the pores of microfluidic reactors are narrow and easy to block, and microorganisms cannot be fixed easily. firm

Method used

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  • Online toxicity monitoring device and monitoring method of bioelectrochemical system
  • Online toxicity monitoring device and monitoring method of bioelectrochemical system
  • Online toxicity monitoring device and monitoring method of bioelectrochemical system

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0140] The monitoring method of the bioelectrochemical system online toxicity monitoring device, the steps are as follows:

[0141] Step 1. Mix 0.8g of sodium acetate, 100mmol of PBS buffer solution, 5mL of vitamins, 12.5mL of trace elements and 335.5mL of activated sludge supernatant to 1L, stir evenly, ventilate nitrogen for more than 40min, airtight, and put it in a biochemical chamber at 38°C Cultivated in the box, after two weeks, the strains were obtained;

[0142] Step 2: Connect the bioelectrochemical system 5 to the electrochemical workstation through wires, inoculate the mixed solution into the bioelectrochemical system 5, place it in a biochemical box at 38°C for cultivation, and replace it when the current collected by the electrochemical workstation drops to 0.00003A For the mixed solution, when the peak value of the current, voltage or electric quantity of the bioelectrochemical system 5 does not increase for two consecutive cycles, it is considered that the bioe...

Embodiment 2

[0162] The device and monitoring method are the same as in Example 1, except that 100 μL of formaldehyde in Example 1 is replaced by 300 μL of formaldehyde.

[0163] After detection, the detection signal is as Image 6 As shown in (a~l), the average inhibition rate is 21.6%.

Embodiment 3

[0165] The device and monitoring method are the same as in Example 1, except that 100 μL of formaldehyde in Example 1 is replaced by 500 μL of formaldehyde.

[0166] After detection, the detection signal is as Figure 7 As shown in (a to p), the average inhibition rate was 36.4%. In order to investigate the recovery of the M3C sensor after being poisoned, seven cycles of 150mg / L sodium acetate solution were passed through, and the current peak value gradually increased (q~w).

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Abstract

The invention discloses an online toxicity monitoring device and monitoring method of a bioelectrochemical system, belongs to the technical field of water body monitoring and provides a device and method of realizing sensitive, quick, low-cost and accurate monitoring of water body toxicity. The monitoring device disclosed by the invention comprises a water sample deoxidizing tank, a cleaning solution deoxidizing tank, a conveying control unit, an acquisition device, a computer and a waste liquid barrel; PLC controls to take a water flow of the bioelectrochemical system from the water sample deoxidizing tank or the cleaning solution deoxidizing tank through a first electromagnetic valve and a second electromagnetic valve; the water flow provides power through a peristaltic pump and squeezesinto the bioelectrochemical system; wastewater discharged by the bioelectrochemical system enters the waste liquid barrel; the acquisition device acquires an electrical signal of the bioelectrochemical system and conveys the electrical signal to the computer to display and store. The monitoring device can realize sensitive, quick, low-cost and accurate monitoring of water body toxicity.

Description

technical field [0001] The invention belongs to the technical field of water body monitoring, and in particular relates to an on-line toxicity monitoring device and a monitoring method for a bioelectrochemical system. Background technique [0002] With the rapid development of industrialization and urbanization in our country, the scope of environmental pollution continues to spread and the degree continues to deepen. Among them, the problem of water pollution is particularly serious. It is of great practical significance to research and develop stable, simple and sensitive online water toxicity monitoring methods and devices. [0003] In recent years, the water toxicity monitoring method based on microorganisms has received extensive attention. The toxicity monitoring results are obtained by directly or indirectly counting the changes in the metabolism or growth rate of a large number of microorganisms. errors caused by body mutations, and are not limited by moral disputes...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N27/416
Inventor 余登斌董绍俊翟俊峰
Owner CHANGCHUN INST OF APPLIED CHEMISTRY - CHINESE ACAD OF SCI
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