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Application of microRNA

A technology of mir-17 and mir-20a, which is applied to alleviate or treat potential substances of bone destruction, screen and prevent microRNA in the field of preparation of drugs that inhibit osteoclast differentiation and bone destruction, and can solve the problem of osteoclasts that has not yet been found. Mechanism of cell action etc.

Inactive Publication Date: 2018-02-16
上海市光华中西医结合医院
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  • Abstract
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  • Claims
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AI Technical Summary

Problems solved by technology

However, the mechanism of action of microRNA on osteoclasts has not yet been found

Method used

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Embodiment Construction

[0027] The present invention discovers the action mechanism of miR-17, miR-20a and miR-92a in the microRNA on osteoclasts by providing an application of microRNA.

[0028] In order to solve the above problems, the main ideas of the embodiments of the present invention are:

[0029] The embodiment of the present invention inhibits osteoclast differentiation microRNA, the microRNA is selected from miR-17, miR-20a and miR-92a, the sequence of the miR-17 is shown in SEQ ID NO: 1, the miR-20a The sequence is shown in SEQ ID NO: 2, which is the sequence of murine mmu-miR-20a-5p; the sequence of the miR-92a is shown in SEQ ID NO: 3, which is the murine mmu-miR-92a- The sequence of 3p.

[0030] The miR-17, miR-20a or miR-92a can be isolated from cells, or can be obtained by artificial synthesis. After knowing the sequences of miR-17, miR-20a, miR-92a or their precursors, those skilled in the art can conveniently prepare and obtain miR-17, miR-20a, miR-92a or their precursors.

[00...

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Abstract

The invention provides application of mircoRNA. The microRNA can be used in the preparation of a medicine for inhibiting osteoclast differentiation and bone destruction. The microRNA is selected frommiR-17, miR-20a and miR-92a. Sequence of the miR-17 is as shown in the SEQ ID NO:1. Sequence of the miR-20a is as shown in the SEQ ID NO:2. Sequence of the miR-92a is as shown in the SEQ ID NO:3. As upregulation of miR-17, miR-20a or miR-92a in the microRNA has an effect of inhibiting osteoclast differentiation, the microRNA can be used as a new therapeutic target for regulating bone metabolism.

Description

technical field [0001] The invention belongs to the field of biotechnology, in particular to the application of a microRNA, in particular to the use of a microRNA in the preparation of drugs for inhibiting osteoclast differentiation and bone destruction, and in screening potential substances for preventing, alleviating or treating bone destruction the use of. Background technique [0002] Bone resorption by osteoclasts plays a key physiological role in the maintenance of bone mineralization homeostasis. Enhanced bone resorption is one of the prominent features of many diseases, such as osteoporosis, bone metastasis of cancer, etc. [0003] Osteoclasts are multinucleated giant cells derived from bone marrow mononuclear cells. First, bone marrow macrophages differentiate into osteoclast precursor cells positive for tartrate-resistant acid phosphatase (TRAP), followed by macrophage colony-stimulating factor (M-CSF) Under the stimulation of receptor activator of nuclear factor...

Claims

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Application Information

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IPC IPC(8): A61K31/7105A61K31/712A61P19/08C12Q1/6883
CPCA61K31/7105A61K31/712C12Q1/6883C12Q2600/136C12Q2600/158C12Q2600/178
Inventor 张昀马晓昀
Owner 上海市光华中西医结合医院
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