Microfluidic chip for quick detection of enzyme-linked immunoassay

Abstract

The invention discloses a microfluidic chip for quick detection of enzyme-linked immunoassay. The microfluidic chip comprises a first chip layer and a second chip layer, at least one detection channelis distributed in the first chip layer, each detection channel is provided with a first sample inlet port, a second sample inlet port and a sample outlet port, and the second sample inlet port is located between the sample outlet port and the first sample inlet port; the second chip layer can move relative to the first chip layer, and a third sample inlet port and a fourth sample inlet port are formed in the second chip layer; when the second chip layer is at the first location, the third sample inlet port is communicated with the first sample inlet port, and the fourth sample inlet port is not communicated with the second sample inlet port; when the second chip layer is at the second location, the third sample inlet port is not communicated with the first sample inlet port, and the fourth sample inlet port is communicated with the second sample inlet port. By the arrangement, the amount of samples used for detection is greatly reduced, and after pretreating and adding of to-be-detected samples for incubation, results can be analyzed quickly by further adding a detection reagent.

Description

technical field [0001] The application belongs to the technical field of enzyme-linked immunoassay rapid detection, in particular to a microfluidic chip for enzyme-linked immunoassay rapid detection. Background technique [0002] The traditional enzyme-linked immunoassay method (ELISA) is operated on a 96-well plate. First, the primary antibody is added to the well plate for incubation. After a certain period of time, the antibody is adsorbed on the surface of the well plate. After washing away the unadsorbed antibody, block the solid surface that does not absorb the primary antibody to reduce non-specific adsorption of proteins. Then, add a standard substance containing a known antigen or a sample to be tested. After incubation for a certain period of time, add a specific enzyme-labeled secondary antibody. After incubation for a certain period of time, the secondary antibody binds to the antigen, and then washes to remove the excess secondary antibody that is not bound. . ...

AI Technical Summary

Problems solved by technology

At present, there is a great demand for ELISA consumables and automatic ELISA in the market. The existing ELISA plate is not only expensive, but also unable to detect the content of multiple target proteins at the same time.

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