Xiangyang radix ophiopogonis endophytic Fusarium sp. fungus and application in steroid saponin preparation

Abstract

The invention discloses Xiangyang radix ophiopogonis endophytic Fusarium sp. fungus and application in steroid saponin preparation, and relates to the technical field of microorganisms. The problems that Xiangyang radix ophiopogonis steroid saponin is mostly directly from Xiangyang radix ophiopogonis extractives, and the Xiangyang radix ophiopogonis steroid saponin is limited in market delivery quantity due to the fact that Xiangyang radix ophiopogonis is long in earthnut production period, low in steroid saponin content and high in saponin extraction cost are solved. The endophytic fungus isseparated from Xiangyang radix ophiopogonis (Liriope spicata var.proliferaY.T.Ma) living earthnuts, it is determined as Fusarium sp. through 18S rDNA sequence authentication, the penicillium oxalicumis preserved in the China Center for Type Culture Collection, the preservation date is 7th December, 2016, and the preservation number is CCTCC No:M2016723. By means of Xiangyang radix ophiopogonis produced in a Xiangyang radix ophiopogonis GAP base-the source area of Xiangyang radix ophiopogonis in Oumiao county, Xiangyang city, Hubei province, due to fermentation of Xiangyang radix ophiopogonisendophytic fungi strain liquid, the Xiangyang radix ophiopogonis steroid saponin is produced. The endophytic fungus is the important microorganism for searching for new resources of the Xiangyang radix ophiopogonis steroid saponin.

Description

technical field [0001] The invention relates to the technical field of microbes, in particular to an endophytic fungus of Xiangmaidong, and the endophytic fungus is an endophytic fungus of the genus Fusarium capable of producing steroidal saponins of Xiangmaidong. Background technique [0002] Xiangmaidong steroidal saponins are national geographical indication products, authentic Chinese medicinal plants in Hubei——Xiangmaidong Liriope spicatavar. prolifera The main active ingredient of Y. T. Ma tuber root, its saponin basic skeleton belongs to the derivative of Spirostane (Spirostane, which has 27 carbon atoms in total), according to the configuration of C25 and the ring closure state of the ring in the Spirostane structure, it can be It is divided into spirostanols (C25 is S configuration), isospirostanols (C25 is R configuration), furostanols (F ring is an open-chain derivative), deformed spirostanols Alcohols (F ring is a five-membered tetrahydrofuran ring) four type...

AI Technical Summary

Problems solved by technology

[0003] Although with the progress of chiral synthesis and oligosaccharide synthesis methods, there have been some reports on the total synthesis of steroidal saponin compounds, but due to the complexity of the steroidal saponin structure, the synthesis is very difficult, and the chemical synthesis is harmful to the environment. Huge pollution, currently mainly obtained directly through the separation of root extracts

However, in the actual production of Ophiopogon japonicus, there are problems such as "small planting area of ​​continuous cropping obstacles", "greatly affected by climatic conditions", "long production cycle of roots", "low content of steroidal saponins", "high cost of saponin extraction". " and other factors have led to restrictions on the market release of xiangmaidong steroidal saponins

At present, there is no report about a kind of endophytic fungus capable of producing xiangmaidong steroidal saponins through microbial fermentation and its application

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