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Small insect hemolymph collecting method

A hemolymph and insect technology, applied in the field of insect physiology and biochemistry, can solve the problems of hemolymph impurity, damage to small insects, pollution, etc., and achieve the effect of ensuring the amount of blood taken and the probability of pollution is small

Active Publication Date: 2018-02-23
TAIYUAN NORMAL UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] At present, hemolymph has become one of the most commonly used research materials in insect research. However, since most insects are small in size and contain little hemolymph, blood coagulation and melanization reactions are prone to occur after in vitro, which brings certain difficulties to the collection work.
[0005] There are many ways to collect hemolymph, such as wax disk method, centrifugation method, dorsal blood vessel method, perfusion method, pressing or homogenization method, shooting bleeding method, tearing method, cutting method, cutting method, puncture method, etc. , but these methods have their own advantages and disadvantages, and are mainly aimed at collecting large and medium-sized insects. For the collection of small insect hemolymph, the body wall or internal structure (such as the intestine) of small insects will often be damaged, resulting in impure or contaminated hemolymph. , and the amount of hemolymph is small, causing difficulties and inconvenience to follow-up research

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0022] A method for collecting small-sized insect hemolymph, comprising the following steps: anesthetizing the worm body, and then separating the thorax and abdomen of the worm body to overflow the hemolymph to obtain the small-sized insect hemolymph.

[0023] Specifically include the following steps:

[0024] (1) Use adhesive tape to fix the centrifuge tube on the table parallel to the vision, and add 0.65% saline dropwise to ensure the physiological activity of the blood;

[0025] (2) Drosophila melanogaster ( Drosophila melanogaster )anaesthetization;

[0026] (3) Place the anesthetized Drosophila melanogaster with its back facing up and laterally at 1 / 3 of the opening of the centrifuge tube, use an insect needle to lightly press the chest of Drosophila melanogaster on the left side, and fix the abdomen of Drosophila melanogaster on the right side , the left and right insect needles form an angle of 45° with the inner wall of the centrifuge tube and the longitudinal axis...

Embodiment 2

[0030] A method for collecting small-sized insect hemolymph, comprising the following steps: anesthetizing the worm body, and then separating the thorax and abdomen of the worm body to overflow the hemolymph to obtain the small-sized insect hemolymph.

[0031] Specifically include the following steps:

[0032] (1) Use adhesive tape to fix the centrifuge tube on the table parallel to the vision, and add 0.65% saline dropwise to ensure the physiological activity of the blood;

[0033] (2) will Anopheles ( Anopheles )anaesthetization;

[0034] (3) Place the anesthetized Anopheles mosquito on the 1 / 3 side of the mouth of the centrifuge tube with its back facing upwards, and use the insect needle to lightly press the Anopheles's chest on the left side and the Anopheles' abdomen on the right side. The inner wall of the tube and the longitudinal axis of the Anopheles body form an angle of 45°;

[0035] (4) Gently pull the left and right insect needles obliquely backward along a 4...

Embodiment 3

[0038] A method for collecting small-sized insect hemolymph, comprising the following steps: anesthetizing the worm body, and then separating the thorax and abdomen of the worm body to overflow the hemolymph to obtain the small-sized insect hemolymph.

[0039] Specifically include the following steps:

[0040] (1) Use adhesive tape to fix the centrifuge tube on the table parallel to the vision, and add 0.65% saline dropwise to ensure the physiological activity of the blood;

[0041] (2) Put sandflies ( Phlebotomus )anaesthetization;

[0042] (3) Place the anesthetized sandfly with its back facing up and laterally at 1 / 3 of the mouth of the centrifuge tube, and use an insect needle to lightly press the chest of the sandfly on the left side and the abdomen of the sandfly on the right side. The needle forms an angle of 45° with the inner wall of the centrifuge tube and the longitudinal axis of the body of the sandfly;

[0043] (4) Gently pull the left and right insect needles...

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PUM

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Abstract

The invention belongs to the field of insect physiology and biochemistry, and particularly relates to a small insect hemolymph collecting method. The small insect hemolymph collecting method can ensure the blood quantity of collected small insects to the maximum extent, and further research is facilitated. By adopting the technical scheme, the small insect hemolymph collecting method comprises thefollowing steps that polypides are narcotized, and then thorax and abdomens of the polypide are separated, so that hemolymph overflows, and the small insect hemolymph is obtained.

Description

technical field [0001] The invention belongs to the field of insect physiology and biochemistry, and in particular relates to a method for collecting small-sized insect hemolymph. Background technique [0002] Insect hemolymph is a mixture of blood and lymph, which circulates in the open circulatory system of insects. It is an important place for insect metabolism and a place for storage and exchange of various substances in the metabolic process. , Damage application and so on play an important role. [0003] Small insects especially Drosophila ( Drosophila ) blood metabolic pathways and hematopoietic mechanisms are highly conserved with mammals. Compared with mammalian models, Drosophila has fewer redundant genes. The study found that insulin and nutrients prevented the differentiation of Drosophila blood stem cells, the inflammatory response caused by dietary changes and the impact on blood development. Type 2 diabetes and obesity are closely linked to chronic inflamma...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N1/10G01N1/28
CPCG01N1/10G01N1/28
Inventor 张晓红石瑛赵文婧郝雪峰郭利敏温海英霍海燕王媚媚
Owner TAIYUAN NORMAL UNIV