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A small insect hemolymph collection method

A hemolymph and insect technology, applied in the field of insect physiology and biochemistry, can solve the problems of damage to small insects, impure hemolymph, and less hemolymph, and achieve the effect of low pollution probability and guaranteed blood volume

Active Publication Date: 2020-08-21
TAIYUAN NORMAL UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] At present, hemolymph has become one of the most commonly used research materials in insect research. However, since most insects are small in size and contain little hemolymph, blood coagulation and melanization reactions are prone to occur after in vitro, which brings certain difficulties to the collection work.
[0005] There are many ways to collect hemolymph, such as wax disk method, centrifugation method, dorsal blood vessel method, perfusion method, pressing or homogenization method, shooting bleeding method, tearing method, cutting method, cutting method, puncture method, etc. , but these methods have their own advantages and disadvantages, and are mainly aimed at collecting large and medium-sized insects. For the collection of small insect hemolymph, the body wall or internal structure (such as the intestine) of small insects will often be damaged, resulting in impure or contaminated hemolymph. , and the amount of hemolymph is small, causing difficulties and inconvenience to follow-up research

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0022] A method for collecting hemolymph of small insects, comprising the following steps: anesthetizing the worm body, then separating the chest and abdomen of the worm body, so that the hemolymph overflows to obtain the small insect hemolymph.

[0023] Specifically include the following steps:

[0024] (1) Fix the centrifuge tube on a table parallel to the vision with tape, and add 0.65% saline to ensure the physiological activity of the blood;

[0025] (2) Drosophila melanogaster ( Drosophila melanogaster )anaesthetization;

[0026] (3) Place the anesthetized Drosophila melanogaster with its back facing up and laterally at 1 / 3 of the centrifuge tube opening, and use an insect needle to gently press on the left side to fix the Drosophila melanogaster chest, and gently press on the right side to fix the Drosophila melanogaster abdomen. , the left and right insect needles form a 45° angle with the inner wall of the centrifuge tube and the longitudinal axis of the Drosophila...

Embodiment 2

[0030] A method for collecting hemolymph of small insects, comprising the following steps: anesthetizing the worm body, then separating the chest and abdomen of the worm body, so that the hemolymph overflows to obtain the small insect hemolymph.

[0031] Specifically include the following steps:

[0032] (1) Fix the centrifuge tube on a table parallel to the vision with tape, and add 0.65% saline to ensure the physiological activity of the blood;

[0033] (2) Anopheles ( Anopheles )anaesthetization;

[0034] (3) Place the anesthetized Anopheles mosquito with its back facing upwards and laterally at 1 / 3 of the centrifuge tube opening. Use an insect needle to gently press on the left side to fix the Anopheles mosquito chest, and gently press to fix the Anopheles mosquito abdomen on the right side. The inner wall of the tube and the longitudinal axis of the body of Anopheles mosquitoes form an angle of 45° respectively;

[0035] (4) Gently pull the left and right insect needl...

Embodiment 3

[0038] A method for collecting hemolymph of small insects, comprising the following steps: anesthetizing the worm body, then separating the chest and abdomen of the worm body, so that the hemolymph overflows to obtain the small insect hemolymph.

[0039] Specifically include the following steps:

[0040] (1) Fix the centrifuge tube on a table parallel to the vision with tape, and add 0.65% saline to ensure the physiological activity of the blood;

[0041] (2) Put the sand flies ( Phlebotomus )anaesthetization;

[0042] (3) Place the anesthetized sandflies with their backs facing upward and laterally at 1 / 3 of the centrifuge tube orifice, and use an insect needle to gently press and fix the chest of the sandflies on the left and the abdomen of the sandflies on the right to fix the insects. The needle forms an angle of 45° with the inner wall of the centrifuge tube and the longitudinal axis of the sandfly body respectively;

[0043] (4) Gently pull the left and right insect ...

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Abstract

The invention belongs to the field of insect physiology and biochemistry, and particularly relates to a small insect hemolymph collecting method. The small insect hemolymph collecting method can ensure the blood quantity of collected small insects to the maximum extent, and further research is facilitated. By adopting the technical scheme, the small insect hemolymph collecting method comprises thefollowing steps that polypides are narcotized, and then thorax and abdomens of the polypide are separated, so that hemolymph overflows, and the small insect hemolymph is obtained.

Description

technical field [0001] The invention belongs to the field of insect physiology and biochemistry, in particular to a method for collecting hemolymph of small insects. Background technique [0002] Insect hemolymph is a mixture of blood and lymph, which circulates in the open circulatory system of insects. It is an important place for insect metabolism and a place where various substances are stored and exchanged during the metabolic process. , damage application and other aspects play an important role. [0003] Small insects, especially fruit flies ( Drosophila ) Blood metabolic pathways and hematopoietic mechanisms are highly conserved with mammals, and Drosophila has fewer redundant genes than mammalian models. The study found that insulin and nutrients prevented Drosophila blood stem cell differentiation, an inflammatory response to dietary changes and an impact on blood development. Type 2 diabetes and obesity are closely linked to chronic inflammation, which is trigg...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N1/10G01N1/28
CPCG01N1/10G01N1/28
Inventor 张晓红石瑛赵文婧郝雪峰郭利敏温海英霍海燕王媚媚
Owner TAIYUAN NORMAL UNIV