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Preparation method and application of polychlorinated biphenyl photoelectrochemical aptamer sensor

An aptamer sensor, polychlorinated biphenyl technology, applied in the direction of material electrochemical variables, scientific instruments, instruments, etc., can solve the problems of poor selectivity, complicated operation, high cost, etc., to achieve increased load capacity, improved sensitivity, and good biological The effect of compatibility

Active Publication Date: 2020-01-03
SHANXI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0004] The purpose of the present invention is to solve the technical problems of high cost, complex operation and poor selectivity of photoelectric analysis when using traditional instrument analysis methods in the detection of polychlorinated biphenyls, and to provide a polychlorinated biphenyl photoelectrochemical aptamer Preparation method and application of sensor

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  • Preparation method and application of polychlorinated biphenyl photoelectrochemical aptamer sensor
  • Preparation method and application of polychlorinated biphenyl photoelectrochemical aptamer sensor
  • Preparation method and application of polychlorinated biphenyl photoelectrochemical aptamer sensor

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Embodiment 1

[0040] A preparation method of a polychlorinated biphenyl photoelectrochemical aptamer sensor in this embodiment comprises the following steps:

[0041] 1. Preparation of nitrogen-doped titania nanotube electrodes:

[0042] 1) Take a titanium plate, polish its surface with metallographic sandpaper after polishing with sandpaper to make the surface of the titanium plate smooth, then ultrasonically clean the titanium plate in high-purity water, acetone and high-purity water for 10-15 minutes, and then use it twice Rinse with distilled water, etch the clean titanium plate in 1:1 diluted concentrated hydrochloric acid at 85°C for 10-15min;

[0043] 2) The etched titanium plate is used as the anode, the Pt sheet is used as the cathode, and the anode titanium plate is placed in a 4 F and 3wt%H 2 O in ethylene glycol solution, anodized at a constant potential of 40-42V for 3 hours to obtain titanium dioxide nanotubes with a titanium plate as the substrate, which were ultrasonically...

Embodiment 2

[0062] A preparation method of a polychlorinated biphenyl photoelectrochemical aptamer sensor in this embodiment comprises the following steps:

[0063] 1. Preparation of nitrogen-doped titania nanotube electrodes:

[0064] 1) Take a titanium plate, polish its surface with metallographic sandpaper after polishing with sandpaper to make the surface of the titanium plate smooth, then ultrasonically clean the titanium plate in high-purity water, acetone and high-purity water for 10-15 minutes, and then use it twice Rinse with distilled water, etch the clean titanium plate in 1:1 diluted concentrated hydrochloric acid at 85°C for 10-15min;

[0065] 2) The etched titanium plate is used as the anode, the Pt sheet is used as the cathode, and the anode titanium plate is placed in a 4 F and 3wt%H 2 O in ethylene glycol solution, anodized at a constant potential of 40-42V for 3 hours to obtain titanium dioxide nanotubes with a titanium plate as the substrate, which were ultrasonically...

Embodiment 3

[0083] A preparation method of a polychlorinated biphenyl photoelectrochemical aptamer sensor in this embodiment comprises the following steps:

[0084] 1. Preparation of nitrogen-doped titania nanotube electrodes:

[0085] 1) Take a titanium plate, polish its surface with metallographic sandpaper after polishing with sandpaper to make the surface of the titanium plate smooth, then ultrasonically clean the titanium plate in high-purity water, acetone and high-purity water for 10-15 minutes, and then use it twice Rinse with distilled water, etch the clean titanium plate in 1:1 diluted concentrated hydrochloric acid at 85°C for 10-15min;

[0086] 2) The etched titanium plate is used as the anode, the Pt sheet is used as the cathode, and the anode titanium plate is placed in a 4 F and 3wt%H 2 O in ethylene glycol solution, anodized at a constant potential of 40-42V for 3 hours to obtain titanium dioxide nanotubes with a titanium plate as the substrate, which were ultrasonically...

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Abstract

The invention relates to a preparation method and an application of a polychlorinated biphenyl photoelectrochemical aptamer sensor. The purpose of the invention is to solve the problems of high cost and complex operation of a traditional apparatus analysis method and the problem of poor selectivity of a traditional photoelectric analysis method in the detection of polychlorinated biphenyls. Chitosan and glutaraldehyde are used to functionalize the surface of a nitrogen-doped titanium dioxide nanotube electrode to modify the surface of the electrode with a polychlorinated biphenyl aptamer; anda DNA-functionalized CdS quantum dot is introduced to the electrode through a base complementation principle to prepare the polychlorinated biphenyl photoelectrochemical aptamer sensor. Compared withexisting analysis techniques, an application method of the aptamer having a specific recognition function, used as an identification element, and the DNA-functionalized CdS quantum dot used as a photoelectric detection signal amplification element in the polychlorinated biphenyl detection has the advantages of high selectivity, high sensitivity, simplicity, easiness in implementation, and can be used to detect the polychlorinated biphenyl in a complex environment system.

Description

technical field [0001] The invention relates to a preparation method and application of a polychlorinated biphenyl photoelectrochemical aptamer sensor. Background technique [0002] Polychlorinated biphenyls (PCBs) are a class of ubiquitous organic pollutants with environmental persistence and bioaccumulation. As early as the 1930s, due to their excellent chemical stability, thermal stability, and low Its flammability and conductivity are mass-produced and widely used in many industrial fields, such as electrical equipment transformers and capacitors, industrial fluids, plasticizers, and insulating oils. However, these substances have serious damaging effects on human health and ecosystems. At present, although most countries have nearly banned the production and use of PCBs, some PCBs will inevitably enter the air, surface water, soil, sediment, etc.; serious threat and damage. Therefore, the rapid and accurate determination of PCBs is of great significance to the protec...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N27/30G01N27/416
CPCG01N27/30G01N27/416
Inventor 范丽芳张彩云史慧杰赵国华
Owner SHANXI UNIV