Method for establishing alcoholic cardiomyopathy C57BL/6 mouse model

Abstract

The invention discloses a method for establishing an alcoholic cardiomyopathy C57BL / 6 mouse model. The method comprises the following steps: A, selecting male C57BL / 6 mice whose weight is about 25 g as objects to establish a mouse model; B, after converting alcohol according to calorie ratio, measuring pharmaceutical 95% alcohol in corresponding volume, and mixing the alcohol with high-fat alcoholliquid fodder, calorie ratios of alcohol in different feeding stages respectively being 5.5%, 11%, 22%, 27%, and 32%, calculating according to 15 ml liquid fodder for each mouse every day, preparingthe liquid fodder when the liquid fodder will be used; C, breeding the selected C57BL / 6 mice in a SPF-level animal house, feeding a model set for 2 days according to a contrast liquid fodder in an adaptive manner, feeding the model set for 2 days using the fodder in which alcohol accounts for 5.5% of calorie ratio, feeding the model set for 2 days using the fodder in which alcohol accounts for 11%of calorie ratio, feeding the model set for a week using the fodder in which alcohol accounts for 22 % of calorie ratio, feeding the model set for a week using the fodder in which alcohol accounts for 27 % of calorie ratio, feeding the model set for a week using the fodder in which alcohol accounts for 32% of calorie ratio, continuously feeding a contrast set (PF) using a contrast liquid fodder for 4 weeks, to make the contrast set and the model set take in fodders in equal calorie, so as to establish the alcoholic cardiomyopathy C57BL / 6 mouse model.

Description

technical field [0001] The invention relates to biotechnology, in particular to a method for establishing a C57BL / 6 mouse model of alcoholic cardiomyopathy. Background technique [0002] Long-term heavy alcohol intake can lead to a non-ischemic form of dilated cardiomyopathy, according to the World Health Organization / International Society and Federation of Cardiology Task Force 1995 report on myocardial The report on the definition and classification of the disease defined it as alcoholic cardiomyopathy (Alcoholic Cardiomyopathy, ACM). At present, the pathogenesis of alcoholic cardiomyopathy is not yet clear. Studies have shown that a large amount of alcohol can cause the loss of left ventricular cardiomyocytes, which in turn can cause cardiac insufficiency, mitochondrial damage, calcium homeostasis imbalance, and changes in myocardial contractile proteins. Since alcoholic cardiomyopathy is a serious threat to human health, further research is needed. Therefore, it is of g...

AI Technical Summary

Problems solved by technology

At present, studies have used alcohol gavage to establish a rat model of alcoholic cardiomyopathy. However, this one-time large amount of alcohol gavage model does not conform to the physiological process of normal people's long-term daily small amount of alcohol intake in daily life, and the gavage operation It will cause different degrees of stress response or death to the animals, which will affect the stability of the test results, and the pathological manifestations will be different, which will limit the application of the model

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