A kind of industrial purification method of fiber grade methanol protein

Abstract

The invention relates to an industrial purification method for fiber-grade methanol proteins and effectively solves the problems of difficult wall breaking of pichia pastoris cells, low breaking rate,easy deactivation and difficult methanol protein purification. The industrial purification method comprises the following steps: performing centrifugal separation on fermentation liquid twice to obtain a heavy phase B; preparing a lysate, adding the lysate in the heavy phase B and adding desalting water to obtain a heavy phase C with a mass concentration of 30 percent; performing high-pressure homogenization to obtain a cell breaking liquid; cooling, regulating a pH value, centrifuging with a centrifugal machine and collecting liquid supernatant to obtain a protein solution; regulating a pH value, performing static precipitation, separating a precipitate with the centrifugal machine and collecting to obtain heavy-phase proteins D; adding desalting water, mixing and centrifuging to obtaineluted re-phase proteins E; regulating pH to obtain a protein alkali solution; drying to obtain the fiber-grade methanol proteins. The industrial purification method provided by the invention has thebenefits that the efficient wall breaking of the pichia pastoris cells as well as the isolation and the purification of the proteins with specific molecular weights are realized, the yield is high, the protein molecular weight distribution is concentrated, the content of crude proteins is above 90 percent, and the requirements for wet spinning of protein fibers can be met.

Description

technical field [0001] The invention relates to the field of protein purification, in particular to an industrial purification method of fiber grade methanol protein. Background technique [0002] Methanol protein is a kind of single-cell protein produced by liquid ventilation fermentation and separation of yeast cells from the fermentation broth with methanol and inorganic salts as the main raw materials. Methanol protein has a crude protein content of more than 50%, balanced amino acids, and high nutritional value, but it cannot be used in feed, food and other industries at present. To develop downstream products of methanol protein and seek new application fields of methanol protein is to solve the problem of methanol overcapacity. It is of far-reaching significance to reduce environmental pollution, develop the national economy, protect the ecological environment and promote the healthy development of related industries. [0003] Methanol protein is a microbial single-c...

AI Technical Summary

Problems solved by technology

[0004] In view of the above situation, in order to overcome the defects of the prior art, the purpose of the present invention is to provide an industrial purification method of fiber-grade methanol protein, which can effectively solve the problem that the cell wall of Pichia pastoris is difficult to break, the fragmentation rate is low, easy to inactivate, and the methanol protein purification is difficult. The problem