Method for extracting and purifying flavonoid components in traditional Chinese medicinal materials

A purification method and technology of flavonoids, which can be applied to medical preparations containing active ingredients, plant/algae/fungus/moss ingredients, pharmaceutical formulations, etc., can solve the problems of low yield, unsuitability, large organic solvents, and high cost, and achieve Effects of improved safety and environmental protection, less organic solvent, and mild reaction conditions

Inactive Publication Date: 2021-02-26
北京美康堂医药科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

So far, methods for enrichment and purification of total flavonoids have been relatively mature, such as high-speed countercurrent chromatography, supercritical carbon dioxide extraction, extraction with agarose gel as a medium, and macroporous adsorption resin purification. Most of these methods have Disadvantages such as high cost, large amount of organic solvent consumption, low output and not suitable for industrial scale
Macroporous adsorption resin is a macromolecular macroporous adsorbent, which shows a unique role in the separation, enrichment and purification of effective components of traditional Chinese medicine. This method is low in cost, easy to regenerate, and consumes less organic solvents. It is suitable for large-scale industrial production, but At present, there are few related studies on the general purification of flavonoids in Chinese herbal medicines, and the specific operation methods and parameters need to be further optimized

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0028] Taking the stem of Astragalus membranaceus as an example to carry out extraction and purification, including the following steps:

[0029] (1) Take the stems of Astragalus membranaceus, wash them, dry them, crush them through a 50-100 mesh sieve, and obtain coarse powder of Astragalus stems;

[0030] (2) Mix the above-mentioned astragalus stem powder with the compound enzymolysis solution at a mass ratio of 1: (8-10), adjust the pH to 4-6, enzymolyze at 45-55°C for 1-3 hours, and then rapidly heat up to 90°C Inactivate the enzyme above ℃, and then thaw the enzymolyzed drug solution. First, pre-freeze the solution at 0-5℃ for 60 minutes, then freeze it at -5℃ for 4 hours, take it out after freezing and thaw it completely at 1-5℃ Then freeze at -10°C for 4 hours, take it out and melt it completely at room temperature, send it to a high-pressure homogenizer, homogenize it at 90-110MPa for 1-3 times, then take it out and filter to obtain the wall-breaking solution;

[0031...

Embodiment 2

[0036] Taking Ficus luteus as an example to carry out extraction and purification, including the following steps:

[0037] (1) Wash and dry Ficus lycens, crush and pass through a 50-100 mesh sieve to obtain Ficus lycens coarse powder;

[0038] (2) Mix the above-mentioned Ficus lyrica coarse powder with the compound enzymolysis solution at a mass ratio of 1: (8-10), adjust the pH to 4-6, enzymolyze at 45-55°C for 1-3 hours, and then rapidly raise the temperature to 90 Inactivate the enzyme above ℃, and then thaw the enzymolyzed drug solution. First, pre-freeze the solution at 0-5℃ for 60 minutes, then freeze it at -5℃ for 4 hours, take it out after freezing and thaw it completely at 1-5℃ Then freeze at -10°C for 4 hours, take it out and melt it completely at room temperature, send it to a high-pressure homogenizer, homogenize it at 90-110MPa for 1-3 times, then take it out and filter to obtain the wall-breaking solution;

[0039] (3) Pass the broken wall liquid through the hig...

Embodiment 3

[0044] Taking kudzu mulberry as an example to extract and purify, it includes the following steps:

[0045] (1) Wash the Pueraria japonica, dry it, and crush it through a 50-100 mesh sieve to obtain Pueraria japonica coarse powder;

[0046] (2) Mix the above-mentioned kudzu powder with compound enzymolysis solution at a mass ratio of 1: (8-10), adjust the pH to 4-6, enzymolyze at 45-55°C for 1-3 hours, and then quickly Raise the temperature to above 90°C to inactivate the enzyme, and then thaw the enzymolyzed medicinal solution. Pre-freeze the solution at 0-5°C for 60 minutes, then freeze at -5°C for 4 hours, and take it out at 1-5°C after freezing. After thawing at ℃, freeze at -10℃ for 4 hours, take it out and thaw at room temperature completely, send it to a high-pressure homogenizer, homogenize it at 90-110MPa for 1-3 times, then take it out and filter to obtain the wall-breaking solution;

[0047] (3) Pass the broken wall liquid through the high-pressure differential conti...

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PUM

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Abstract

The invention discloses a method for extracting and purifying flavonoid components in traditional Chinese medicinal materials. The method comprises the following steps of: (1) cleaning traditional Chinese medicinal materials, drying, performing pulverizing, and sieving through a 50-100-mesh sieve to obtain crude medicinal material powder; (2) performing wall breaking on the crude medicinal material powder by taking water as a solvent to obtain wall-broken liquid; (3) performing primary separation and concentration on the wall-broken liquid through high-pressure-difference continuous extractionequipment, and centrifugally collecting clear liquid for later use; and (4) adjusting the pH value of the clear liquid to 4.5-5.5, and purifying with macroporous resin to obtain a high-purity flavonoid component extracting solution. The extraction and purification method disclosed by the invention adopts a combined process, is scientific and reasonable, precise in process parameter, less in usedorganic solvent and milder in reaction condition, and can adapt to industrial production, so that the method has an ideal development prospect.

Description

technical field [0001] The invention belongs to the technical field of extracting traditional Chinese medicines, in particular to a method for extracting and purifying flavonoids in Chinese medicinal materials. Background technique [0002] Flavonoids are a class of natural products widely distributed in Chinese herbal medicines. At present, flavonoids are extracted from Chinese herbal medicines, and commonly used methods include hot water extraction, organic solvent extraction, ultrasonic-assisted extraction, microwave-assisted extraction, and supercritical fluid extraction. Among them, the hot water extraction method is generally limited to the extraction of flavonoid glycosides. This process has simple equipment, low cost, and is non-toxic to the environment and humans. It is suitable for industrial production, but the extraction time is long and the extraction rate is low. The organic solvent extraction method selects a suitable organic solvent according to the differen...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K36/481A61K36/60
CPCA61K36/481A61K36/60A61K2236/19A61K2236/331A61K2236/39A61K2236/55
Inventor 王亚平杨百艳胡颖高峰杨丹丹豆环宇郑冬梅
Owner 北京美康堂医药科技有限公司
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