Biological demoulding agent and preparation method

A mold remover, biological technology, applied in animal feed, animal feed, application and other directions, can solve the problems of restricting large-scale application and promotion, high price, and achieve the effect of simple and flexible use, low price, and protection of intestinal mucosa

Active Publication Date: 2018-03-30
DALIAN SANYI ANIMAL MEDICINE CO LTD +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

But the strain can only degrade aflatoxin, and the price is high, which limits its large-scale application and promotion

Method used

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  • Biological demoulding agent and preparation method
  • Biological demoulding agent and preparation method
  • Biological demoulding agent and preparation method

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0021] The biological mold removal agent of the present invention is formed by mixing and drying the Bacillus subtilis / yeast cell wall conjugate and glucose. The colonization time of the Bacillus subtilis in the digestive tract of the host is at least 12 hours. The mass ratio of the compound to the glucose is 1:15.

[0022] Follow the steps below to prepare:

[0023] a. Inoculate the seed solution of Bacillus subtilis (Accession No.: CICC 20683) that has been colonized in the digestive tract of the host for at least 12 hours in a 1:100 (VN) ratio in a 1000 mL / 5000 mL Erlenmeyer flask filled with medium, 37 Shake culture at ℃ overnight, after the strain grows to the late logarithmic stage, centrifuge to collect the bacterial pellet, then wash with sterile PBS (pH 8.0) for 2-3 times, and finally use an appropriate volume of PBS buffer (pH 8.0) to make a bacterial suspension for later use ;

[0024] b. Add yeast cell wall (MOS) to the bacterial suspension according to the ratio...

Embodiment 3

[0061] The biological mildew remover of the present invention is formed by mixing and drying the Enterococcus faecium / yeast cell wall conjugate and soluble starch. The colonization time of the Enterococcus faecium in the digestive tract of the host is at least 48 hours. The mass ratio of conjugate to soluble starch is 1:25.

[0062] Follow the steps below to prepare:

[0063] a. Inoculate the seed solution of Enterococcus faecium (Accession No.: CICC 6078) that has been colonized in the digestive tract of the host for at least 48 hours in a 1:100 (VN) ratio in a 1000 mL / 5000 mL Erlenmeyer flask filled with medium, 37 Shake culture at ℃ overnight, after the strain grows to the late logarithmic stage, centrifuge to collect the bacterial pellet, then wash with sterile PBS (pH 8.0) for 2-3 times, and finally use an appropriate volume of PBS buffer (pH 8.0) to make a bacterial suspension for later use ;

[0064] b. Add yeast cell wall (MOS) to the bacterial suspension according t...

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Abstract

The invention discloses a biological demoulding removal agent. The biological demoulding removal agent is prepared by mixing and drying probiotics / yeast cell wall conjugate and carrier, wherein the planting time of the prebiotics in the host digestion tract is at least 12h; the mass ratio of the probiotics / yeast cell wall conjugate to the carrier is 1:(5 to 25). A preparation method sequentially comprises the following steps of selecting the prebiotics of which the planting time in the host digestion tract is at least 12h, expanding and culturing, and using a PBS (phosphate buffer saline) buffer solution to prepare a bacterial suspension; adding yeast cell wall into the bacterial suspension according to the mass ratio of the bacterial suspension to the yeast cell wall equal to (2 to 10):1,and measuring the total content of protein; adding carbodiimide, slowly stirring, and reacting and protecting from light for 8 to 24h, wherein the mass ratio of the total protein to the carbodiimideis (0.2 to 10):1; centrifuging a reaction solution, removing supernatant, adding normal saline into precipitate, and dissolving precipitate; adding the carrier, mixing, and drying into powder, whereinthe mass ratio of the precipitate to the carrier is 1:(5 to 25).

Description

technical field [0001] The invention belongs to the field of feed additives, in particular to a cheap and efficient biological mold remover capable of stably adsorbing various mycotoxins in feed and a production method thereof. Background technique [0002] Mycotoxins are a class of toxic and harmful substances produced by molds or other fungi that are widely present in crops, food and feed (Darwish WS, et al., 2014). For the livestock and poultry breeding industry, it can damage the liver and kidneys of animals, seriously hinder the normal metabolism and immune function of animals, and some can even cause cancer and teratogenicity (Awad W, et a1., 2013). During the collection of feed materials, product processing, storage and transportation, and sales and use, they are inevitably polluted by molds and their toxins. It can be said that mycotoxins are everywhere (Tang Shaowei et al., 20l0). Among them, the threat of aflatoxins to human and animal health is well known (Gunthe...

Claims

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Application Information

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IPC IPC(8): A23K10/16A23K10/18
CPCA23K10/16A23K10/18
Inventor江国托刘艳林洋王效禹单春乔王杲强
OwnerDALIAN SANYI ANIMAL MEDICINE CO LTD