Preparation method of a ratiometric electrochemical dopamine aptamer sensor
An electrochemical dopamine aptamer and sensor technology, which is applied in the field of sensors and nanomaterials intersecting electrochemical analysis and biological sciences, can solve the problems of no sensor preparation process and no detection of dopamine, and achieve simple preparation process and low preparation cost The effect of low and high product sensitivity
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Embodiment 1
[0025] In this example, the preparation of a ratiometric electrochemical dopamine aptasensor based on the aptamer-gold nanoparticles / reduced graphene oxide-Nile blue nanocomposite and the principle schematic diagram of the ratiometric electrochemical signal detection of dopamine can be found in figure 1 As shown, add 1.0 g of graphite to the three-necked flask, add 25 ml of concentrated sulfuric acid carbonized graphite dropwise, stir slowly for 24 hours, add 1.5 g of potassium permanganate, transfer the three-necked flask to an ice bath for cooling, continue to stir for 30 minutes, and heat up to 60°C, stirring and reacting for 45 minutes, adding 3 milliliters of distilled water every 15 minutes, adding 180 milliliters of distilled water to terminate the reaction after the reaction, the product was cooled to room temperature, filtered, washed with distilled water, and dried to obtain graphene oxide; Under the action of ultrasound, graphene oxide was dispersed in distilled wate...
Embodiment 2
[0027]The present embodiment adopts the Hummers method to prepare graphene oxide, and the specific method is the same as in Example 1, and then the graphene oxide is dispersed in distilled water under the action of ultrasound, and then Nirlan is added to form a mixed solution, wherein graphene oxide and Nerlan The mass concentration ratio is 1:1, and the mixed solution is drop-coated on the surface of a newly polished glassy carbon electrode at room temperature to prepare a graphene oxide-Nierlan modified glassy carbon electrode; the modified glassy carbon electrode is inserted into a gold chloride In the phosphate electrolyte with acid as the electrolyte, scan at a certain voltage for a period of time, so that gold nanoparticles can be electrodeposited on the surface of the glassy carbon electrode, and graphene oxide is electroreduced into reduced graphene oxide at the same time, where the scan voltage is - 1.5V, scanning time 120 seconds; through the Au-S bond, the single str...
Embodiment 3
[0029] The present embodiment adopts the Hummers method to prepare graphene oxide, and the specific method is the same as in Example 1, and then the graphene oxide is dispersed in distilled water under the action of ultrasound, and then Nirlan is added to form a mixed solution, wherein graphene oxide and Nerlan The mass concentration ratio is 1:2, and the mixed solution is drop-coated on the surface of a newly polished glassy carbon electrode at room temperature to prepare a graphene oxide-Nierlan modified glassy carbon electrode; the modified glassy carbon electrode is inserted into a gold chloride In the phosphate electrolyte with acid as the electrolyte, scan at a certain voltage for a period of time, so that gold nanoparticles can be electrodeposited on the surface of the glassy carbon electrode, and graphene oxide is electroreduced into reduced graphene oxide at the same time, where the scan voltage is - 0.5V, scanning time 80 seconds; through the Au-S bond, the dopamine a...
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