Programmatic isothermal nucleic acid amplification method and applications of kits of isothermal nucleic acid amplification method

A constant-temperature amplification and nucleic acid technology, which is applied in the fields of biochemical equipment and methods, and the determination/inspection of microorganisms, can solve the problems of dependence on instruments and equipment, time-consuming and laborious, and complicated steps, and achieve the effect of rapidly amplifying target sequences.

Pending Publication Date: 2018-04-13
江苏达伯药业有限公司
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0008] The present invention aims at the deficiencies of time-consuming and laborious nucleic acid molecular biology detection in the field of biotechnology, cumbersome steps and dependence on instruments and equipment, and aims to provide people with a simple and cheap nucleic acid constant temperature detection method, so that Advanced molecular detection methods can be widely promoted and applied effectively in on-site detection events and grassroots community hospitals

Method used

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  • Programmatic isothermal nucleic acid amplification method and applications of kits of isothermal nucleic acid amplification method
  • Programmatic isothermal nucleic acid amplification method and applications of kits of isothermal nucleic acid amplification method
  • Programmatic isothermal nucleic acid amplification method and applications of kits of isothermal nucleic acid amplification method

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0102] Example 1. Test kit for Mycobacterium tuberculosis

[0103] Tuberculosis (M. Tuberculosis, TB) is an infectious disease that seriously endangers human health. It is a major problem that hinders the country's economic and social development. It is one of the major diseases that our country focuses on control, and it is also a public health and social problem of global concern. In recent years, the global tuberculosis epidemic has rebounded, which has attracted great attention from the international community. The World Health Organization has made tuberculosis one of the most important infectious diseases to be controlled, and called for new diagnostic tools targeting low- and middle-income countries. Industrial investment.

[0104] At present, there are many techniques used in tuberculosis diagnosis. Although these routine examination methods are technically mature, there are still some problems: the internationally recommended smear and culture methods also have poor specif...

Embodiment 2

[0127] Example 2. Detection of amplification products of Mycobacterium tuberculosis

[0128] In the reaction system of Example 1, calcein was used to replace EvaGreen and other reaction conditions were maintained unchanged, and then a handheld purple light source and a gel electrophoresis system were used to detect constant temperature amplification products. The test results show that Image 6 In B.

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Abstract

The invention relates to a method for amplifying and detecting a target nucleic acid sequence under an isothermal condition by virtue of relates specific primers. The method provided by the invention,through the design of the special primers, can fundamentally achieve a purpose of implementing rapid, convenient, efficient and specific amplification and detection on target genes under the isothermal condition. Meanwhile, the invention also relates to applications of related kits in the aspect of rapid nucleic acid diagnosis, wherein the applications include an application in the aspect of detection of such pathogenic microorganisms as bacteria, germs and the like, and an application in diagnosis of related genes of human genetic diseases.

Description

Technical field [0001] The present invention relates to a nucleic acid molecular biology detection method in the field of biotechnology, in particular to a programmed nucleic acid constant temperature amplification method and its kit application. Background technique [0002] According to the World Health Organization’s global epidemiological trend survey research, the previous conventional pathogen detection methods (such as bacterial culture, external morphological structure, physiological and biochemical identification, and serological identification, etc.) are time-consuming and labor-intensive, steps are cumbersome, and the instruments and equipment Insufficient dependence, etc., has been unable to effectively meet the requirements of humans for rapid and specific detection of pathogenic microorganisms. The rapid development of molecular biology technology allows people to quickly / correctly identify pathogenic microorganisms that are difficult to detect by conventional detec...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/6844
CPCC12Q1/6844C12Q2531/119C12Q2563/107
Inventor 徐高连单洪波樊春海侯建华
Owner 江苏达伯药业有限公司
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