Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

An electrochemical DNA biosensor for gender and variety identification

A biosensor, electrochemical technology, applied in the field of biosensors, can solve the problem of high labor intensity

Inactive Publication Date: 2018-04-17
NATIONAL UNIVERSITY OF MALAYSIA +1
View PDF8 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Furthermore, these techniques are too slow and labor intensive and can only be performed in the laboratory

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • An electrochemical DNA biosensor for gender and variety identification
  • An electrochemical DNA biosensor for gender and variety identification
  • An electrochemical DNA biosensor for gender and variety identification

Examples

Experimental program
Comparison scheme
Effect test

example

[0040] 1. Selection of oligonucleotide sequence (DNA probe)

[0041] Oligonucleotide sequences for multiple probes (which are 2 to 3 different probes) for determinations involving sex and class will be involved in sex or class determination. These probes were obtained by molecular techniques (PCR, cloning and electrophoresis) on more than one hundred dragonfish tissue samples. The two to three probes with the best specificity for Arowana sex or species were selected and then used for biosensor fabrication. These probes are 20 to 25 bp long.

[0042] 2. Preparation of DNA biosensor based on silica nanosphere composite

[0043] like figure 1 The three-electrode system of the electrochemical DNA biosensor electrode (100) shown in is used for DNA biosensor measurements, with a carbon paste screen-printed electrode (Scrint Technology (M)) as the working electrode (102), as a reference electrode ( 106) of the printed Ag / AgCl electrode, and carbon paste counter electrode (104). ...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

An electrochemical DNA biosensor comprising a screen-printed electrode modified with gold nanoparticles, a layer of silica nanosphere composite, an oligonucleotide sequence, a cross-linking agent anda redox indicator. The layer of silica nanosphere composite is deposited on the screen-printed electrode and the oligonucleotide sequence is immobilized on the layer of silica nanosphere composite using the cross-linking agent. Further, at least a portion of the oligonucleotide sequence is complementary to a unique target sequence of a nucleic acid from a fish of interest such that the nucleic acid from the fish of interest hybridizes to the oligonucleotide sequence to form a dsDNA. Furthermore, the redox indicator intercalates with the dsDNA which enables detection of gender and variety of the fish by voltammetry.

Description

technical field [0001] Embodiments of the present invention relate to biosensors, and more particularly to electrochemical DNA biosensors that rapidly identify sample DNA for identifying sex and type of arowana without requiring any skilled operator. In addition, it can be miniaturized according to the needs of use. In addition, it offers the advantage of a portable and user-friendly structure. Background technique [0002] In recent years, the demand for Malaysian Arowana (Scleropages formosus) has been increasing worldwide. This expensive ornamental fish is endemic to Malaysia and up to 60,000 fish are exported every year with an average price of RM10,000 per fish. Therefore, it is important to identify this species due to its high demand. Therefore, research and development of a portable, easy-to-use, and low-cost bioanalysis system for rapidly diagnosing the sex and type of these fish has been actively performed. Among these systems, DNA hybridization-based biosensor...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/6888G01N27/327G01N33/487
CPCC12Q1/6816G01N27/3276G01N27/3278C12Q2565/519C12Q2565/607
Inventor 李毓兴宗可辉阿布德拉希德刘宝嫱德迪弗特拉
Owner NATIONAL UNIVERSITY OF MALAYSIA
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com