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A kind of ultra-low temperature preservation method of mango pollen non-contact mesh bag

A low-temperature storage and non-contact technology, which is applied in the fields of botanical equipment and methods, fruit hanging devices, agricultural machinery and implements, etc., can solve problems such as failure of flowering period, and achieve the effect of maintaining vitality, reliable effect, and simple and easy method

Active Publication Date: 2021-02-12
YUNNAN INST OF TROPICAL CROPS
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] The main problem to be solved by the present invention is to disclose a non-contact net bag ultra-low temperature storage method for mango pollen.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0020] 1. Prepare a liquid nitrogen tank and liquid nitrogen according to the above technical scheme; prepare a rectangular nylon mesh bag with a pore size of 10 μm and a size of 2 cm×4 cm.

[0021] 2. The non-contact net bag ultra-low temperature pollen preservation method of the present invention was implemented to the mango cultivar 'Nannianmang'.

[0022] 3. Collect the mature anthers of 'Three Years' when there is no dew, place them in a white shallow porcelain plate with a diameter of 10 cm, and dry them in a constant temperature drying oven at 34°C until the water content is 7.0-7.5%, shake out the pollen with tweezers, pick Remove impurities.

[0023] 4. Put the dried 'Nannianmang' pollen into the prepared nylon mesh bag, shake gently so that the pollen of 'Nannianmang' is placed at the bottom of the mesh bag, and then clamp the edge of the mesh bag with tweezers The alcohol lamp gently burns the seal.

[0024] 5. Tie and seal the nylon mesh bag with ‘Three Years of ...

Embodiment 2

[0030] 1. Prepare a liquid nitrogen tank and liquid nitrogen according to the above technical scheme; prepare a rectangular nylon mesh bag with a pore size of 10 μm and a size of 2 cm×4 cm.

[0031] 2. The non-contact net bag ultra-low temperature pollen preservation method of the present invention was implemented to the mango wild germplasm 'YONGDE-1'.

[0032] 3. Collect mature anthers of 'YONGDE-1' when there is no dew, place them in a white shallow porcelain plate with a diameter of 12cm, and dry them in a constant temperature drying oven at 36°C until the water content is 6.5-7.0%. Shake out the pollen with tweezers, pick Remove impurities.

[0033] 4. Put the dried 'YONGDE-1' pollen into the prepared nylon mesh bag, shake gently so that the 'YONGDE-1' pollen is placed at the bottom of the mesh bag, and then clamp the edge of the mesh bag with tweezers The alcohol lamp gently burns the seal.

[0034] 5. Tie and seal the nylon mesh bag with 'YONGDE-1' pollen with a thin ...

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PUM

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Abstract

The invention belongs to the pollen preservation technology of agricultural and forestry economic crops, and in particular relates to a method for ultra-low temperature preservation of mango pollen in a non-contact mesh bag. The present invention consists of the following steps: using mango pollen collected when there is no dew, drying to a water content of 6.5-7.5%; using a mesh bag with a diameter smaller than the pollen diameter to hold the pollen, and placing it in the upper space of a liquid nitrogen tank for 28-33 day; take out the pollen and thaw it in an artificial climate box with a humidity of 60-65% and a temperature of 10-15°C, and then incubate in an artificial climate box with a humidity of 88-92% and a temperature of 34-36°C for 40-45 minutes, and then it can be used Vigor assays and cross pollination. Adopting the invention can prolong the maintenance time of mango pollen vigor from several hours normally to one month without causing pollen rupture or internal structure damage.

Description

technical field [0001] The invention belongs to the pollen preservation technology of agricultural and forestry economic crops, and in particular relates to a method for ultra-low temperature preservation of mango pollen in a non-contact mesh bag. [0002] technical background: [0003] mango (Mangifera indica L.) is Anacardiaceae (Anacardiaceae) Mango ( Mangifera ) Tall trees, which have a long history of cultivation and artificial cultivation for more than 4,000 years. They are cultivated in a wide range of regions and are distributed in more than 100 tropical and subtropical countries in the world. Because of its beautiful fruit shape, sweet taste, strong fragrance and rich nutrition, it is called "the king of tropical fruits". The main producing areas of mango in the world are India, Indonesia, Malay Peninsula, Thailand and other Southeast Asian countries and regions, and there are more than 300 varieties grown on a large scale. Mango planting is an important part of...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01F25/00
CPCA01F25/00
Inventor 柳觐孔广红牛迎凤刘紫艳
Owner YUNNAN INST OF TROPICAL CROPS