A method for preparing high-purity mogroside V

Abstract

The invention provides a method for preparing high-purity mogroside V, and belongs to the technical field of extraction of effective components in natural plant. The method comprises the following steps: pretreatment of raw materials, countercurrent extraction, multistage filtering, strong-base anion resin decolorization, concentration, vacuum concentration and freeze drying. The method combines the characteristics of multistage filtering, realizes continuous extraction, is simple in process and suitable for industrial large scale production. Efficient liquid detection shows that mogroside V of a product prepared through the method is 65 to 68 percent, the yield is 82 to 86 percent, the formed mogroside V is high in purity, organic solvents are not used during extraction, the product is safe, and the quality is guaranteed.

Description

technical field [0001] The invention belongs to the technical field of extracting active ingredients from natural plants, and in particular relates to a method for preparing high-purity mogroside V. Background technique [0002] Luo Han Guo is the fruit of perennial herbaceous vines belonging to Cucurbitaceae. It is a unique plant in southern my country. It is mainly produced in northern Guangxi. The quality of Luo Han Guo in Guilin, Guangxi is the best. Luo Han Guo has been eaten as traditional Chinese medicine and dried fruit for a long time. It tastes extremely sweet, cool in nature, non-toxic, and enters the spleen and lungs. Quenches thirst, promotes gastrointestinal function, suppresses asthma and lowers blood pressure. Since it was first reported in 1975 that the fruit of Luo Han Guo contained diterpene sweeteners, mogroside IV (mogroside IV), mogroside V, mogroside III, mogroside IIE, mogroside IIIE, mogroside VI, mogroside A, mogroside (nemogroside), siamenside I ...

AI Technical Summary

Problems solved by technology

[0003] There are many existing methods for extracting mogroside, but the purity of mogroside V in the obtained extracts is relatively low, most of which do not exceed 80%. CN101863946A patent discloses a method for extracting high-content mogroside V. The patent mentions that Luo Han Guo is crushed and extracted with alcohol, concentrated, separated by macroporous adsorption resin, decolorized by ion exchange resin, and finally dried to obtain the finished product. The process is simple, but the purity of the product is low

CN101690573A patent discloses a method in which mogroside V can be greater than 60%, mainly adopts enzymatic hydrolysis, water extraction, concentration, ion exchange resin decolorization, macroporous adsorption resin separation and alumina chromatography, and finally concentrates and dries to obtain the finished product The method, this method is through multi-step chromatography, causes the yield of final product to be lower

CN10140266A patent discloses the preparation method of mogroside V, through silica gel chromatography and C18 reverse phase chromatography, the purity of the final product obtained can reach more than 70%, but more organic solvents are used in the process, and the cost is high. Not suitable for industrial production

CN104086615A patent discloses a method for extracting mogroside V, which uses a supercritical fluid extraction method. Although the purity of the final product is also above 70%, the limitation of the processing capacity of supercritical fluid extraction equipment is not conducive to large-scale production