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Method for extracting kolavenic acid from polyalthia longifolia var.prndulla R.Br.

A technology of cosulfuric acid and sulfamethoxazole, which is applied in the field of medicine, can solve the problems such as undiscovered public reports of cosulfuric acid and few studies of cosulfuric acid, and achieves the effects of stable process, continuous extraction and solvent saving.

Inactive Publication Date: 2012-09-26
NANJING ZELANG MEDICAL TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] At present, there are few studies on kolavulanic acid, and no public reports on industrialized methods for preparing kolavulanic acid have been found.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0013] Crush the bark of the drooping Anthera longifolia stem, weigh 1000g, put it into the extraction kettle, and feed it with CO in a supercritical state. 2 For extraction, set the parameters of the extraction kettle: the extraction temperature is 38°C, the pressure is 20MPa, and CO 2 The flow rate is 18L / h. After the environment in the extraction tank is stabilized, 50ml of ethanol is introduced as an entrainer to participate in the extraction. After 2 hours of extraction, the oily extract is collected from the separation kettle, and the extract is extracted with ether repeatedly for 4 times, and the ether extract is combined. , After concentration, ether extract was obtained. Mix the ether extract with an appropriate amount of magnesium oxide and mix well, pack the column with 5 times the amount of magnesium oxide of the extract (column diameter-to-height ratio 1:10), place the mixed sample magnesium oxide on the top of the column, and use n-hexane-ether by volume Gradien...

Embodiment 2

[0015] Crush the bark of the drooping Anthera longifolia stem, weigh 1000g, put it into the extraction kettle, and feed it with CO in a supercritical state. 2 For extraction, set the parameters of the extraction kettle: the extraction temperature is 60°C, the pressure is 28MPa, and CO 2 The flow rate is 15L / h. After the environment in the extraction tank is stabilized, 150ml of ethanol is introduced as an entrainer to participate in the extraction. After 2 hours of extraction, the oily extract is collected from the separation kettle, and the extract is repeatedly extracted with ether for 4 times, and the ether extract is combined. , After concentration, ether extract was obtained. Mix the ether extract with an appropriate amount of magnesium oxide and mix well, pack the column with 5 times the amount of magnesium oxide of the extract (column diameter-to-height ratio 1:8), place the mixed sample magnesium oxide on the top of the column, and use n-hexane-ether by volume Gradien...

Embodiment 3

[0017] Crush the bark of the drooping Anthera longifolia stem, weigh 1000g, put it into the extraction kettle, and feed it with CO in a supercritical state. 2 For extraction, set the parameters of the extraction kettle: the extraction temperature is 40°C, the pressure is 35MPa, and CO 2 The flow rate is 20L / h. After the environment in the extraction tank is stabilized, 100ml of ethanol is introduced as an entrainer to participate in the extraction. After 1 hour of extraction, the oily extract is collected from the separation kettle, and the extract is extracted with ether repeatedly for 3 times, and the ether extract is combined. , After concentration, ether extract was obtained. Mix the ether extract with an appropriate amount of magnesium oxide and mix well, use 5 times the amount of magnesium oxide of the extract to pack the column (the ratio of column diameter to height is 1:15), put the mixed sample magnesium oxide on the top of the column, and use n-hexane-ether by volum...

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PUM

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Abstract

The invention provides a method for extracting kolavenic acid from polyalthia longifolia var.prndulla R.Br. The method comprises the following steps of crushing root barks of polyalthia longifolia var.prndulla R.Br., feeding into an extraction kettle, introducing CO2 in a supercritical state and extracting to obtain an oily extract, extracting the extract by using diethyl ether for multiple times, concentrating the obtained extract liquor to obtain diethyl ether extract, adding the diethyl ether extract into a magnesium oxide column for chromatography, performing gradient elution by using normal hexane and diethyl ether, performing rotary evaporation on eluent to concentrate, drying to obtain a kolavenic acid crude product, and separating by preparative high performance liquid chromatography to obtain the kolavenic acid. The process is simple, the yield is high, and a product is high in quality and purity.

Description

technical field [0001] The invention belongs to the technical field of medicines, and relates to a method for extracting kolavulanic acid from Anthropomorpha pendula. Background technique [0002] Colavulanic acid is a diterpenoid with the molecular formula C 20 h 32 o 2 , with a molecular weight of 304.47, is mainly found in the stem bark of Annonaceae (Annonaceae) and is rich in resources. Pharmacological studies have shown that kolavonic acid has cytotoxic activity, has significant inhibitory effect on Crown gall tumors, and has obvious effects on three human tumor cell lines (A-549 lung cancer, MCF-7 breast cancer, HT-29 colon adenocarcinoma). Cytotoxic activity; antibacterial activity, strong inhibitory effect on Gram-positive bacteria (Bacillus subtilis and Staphylococcus aureus); antifeedant activity. At 1% concentration, it has strong antifeedant activity against subterranean termites. [0003] At present, there are few studies on kolavulanic acid, and no public...

Claims

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Application Information

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IPC IPC(8): C07C57/26C07C51/47C07C51/42
CPCY02P20/141Y02P20/54
Inventor 刘东锋杨成东
Owner NANJING ZELANG MEDICAL TECH
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