Endoglucanase, its coding gene cel5a-h31 and its application

An endoglucanase and gene technology, applied in the field of genetic engineering, can solve problems such as slow process, achieve the effects of short production cycle, strong pH tolerance, and ensure amplification efficiency
CN107937371BInactive Publication Date: 2019-06-11ZHEJIANG UNIV
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Patents(China)
Current Assignee / Owner
ZHEJIANG UNIV
Publication Date
2019-06-11
Estimated Expiration
Not applicable · inactive patent

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Abstract

The invention belongs to the field of genetic engineering and in particular relates to novel endoglucanase, coding genes cel5A-h31 and application thereof. A nucleotide sequence of genes cel5A-h31 coding the endoglucanase is shown as SEQ ID No. 1. Bioinformatics analysis shows that the endoglucanase coded by the genes belongs to the fifth family. The endoglucanase is successfully cloned by the inventor by virtue of a design primer and successfully expressed in a prokaryotic expression system, the recombinant endoglucanase obtained by induced ultrasonic purification has an optimal operating pHvalue of 5.0 and an optimal temperature of 55 DEG C, can maintain relatively stable enzymatic activity at a temperature of 50 DEG C or below, has high pH tolerance and can maintain the enzymatic activity of 75% or higher at the pH value of 5.0-9.0. The recombinant endoglucanase has high research and industrial application potential.
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Description

technical field

[0001] The invention belongs to the field of genetic engineering, and specifically relates to a novel endoglucanase, its coding gene cel5A-h31 and application thereof. Background technique

[0002] As the main component of plant cell walls, cellulose is currently the most abundant renewable resource in nature, but its stubborn structure limits its utilization. The process of degrading cellulose into glucose requires endoglucanase (endoglucanase, EC3.2.1.4), exoglucanase (exoglucanase, E.C.3.2.1.91) and β-glucosidase (β-glucosidase, E.C. 3.2.1.21) The three cellulase systems act together. Among them, endoglucanase is the most important component of the cellulase system, it mainly acts on the non-crystalline region of cellulose, randomly hydrolyzes the β-1,4 glycosidic bonds in the cellulose chain, cuts off the long chain of cellulose, It is converted into a large number of short chains with different degrees of polymerization, reducing the degree of polymeri...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
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