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Method for cloning dog by adopting somatic cells

A technology of somatic cell cloning and somatic cells, which is applied in the field of improved somatic cell cloning of dogs, can solve the problems of increased production costs, low success rate, and difficult industrial application of cloning dogs, so as to improve implantation efficiency and pregnancy efficiency , Improve the effect of fusion efficiency

Pending Publication Date: 2018-04-20
BEIJING SINOGENE BIOTECHNOLOGY CO LTD
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Problems solved by technology

[0006] However, in the existing technology of somatic cell cloning of dogs, female dogs in estrus at the same time are often selected for allogeneic embryo transfer, and the success rate is low, resulting in an increase in the production cost of cloned dogs. Many, complex operation means, difficult to achieve industrial application

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  • Method for cloning dog by adopting somatic cells
  • Method for cloning dog by adopting somatic cells
  • Method for cloning dog by adopting somatic cells

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Embodiment

[0036] (1) Isolation and culture of somatic cells

[0037]In this experiment, the somatic cell donor dog is the experimental Beagle-Apple (No. 161207) raised by Beijing Sino Valley Biotechnology Co., Ltd., which is the world's first APOE gene prepared by Beijing Sino Valley Biotechnology Co., Ltd. Knockout dogs (the classification with all its genetic information is named apolipoprotein E (APOE) gene knockout Beagle ear fibroblasts: BGD-APOEKO-EF0 is preserved in the General Microbiology Center of China Microbiological Culture Collection Management Committee ( CGMCC), the preservation address is: No. 3, No. 1 Courtyard, Beichen West Road, Chaoyang District, Beijing (postal code 100101), the preservation number is CGMCC No.13804, and the preservation date is March 1, 2017). When taking skin tissue, first use a razor to scrape off the fur around the dog’s ear margin tissue to be collected, disinfect with 75% alcohol, and cut about 1 cm of the dog’s ear margin tissue with sterili...

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Abstract

The invention relates to a method for cloning a dog by adopting somatic cells, and particularly relates to a method for preparing a somatic cell cloned dog by adopting a low osmotic pressure fusion solution and an autologous embryo transplantation technology.

Description

technical field [0001] The present invention relates to an improved method for somatic cell cloning of dogs, in particular to a method for preparing somatic cell cloning of dogs by adopting low osmotic pressure fusion solution together with autologous embryo transfer technology. Background technique [0002] Animal cloning technology is to transfer cells into recipient oocytes through nuclear transfer to produce animal individuals with the same DNA sequence information as the donor cells. The biggest feature of this technology is that the cloned animals born have the same genetic information as the donor cells. Therefore, cloning technology can be used to replicate animal cells, and cloning technology can be applied to the production of transgenic animals, the propagation of high-quality livestock, the preservation of endangered animal resources, and therapeutic cloning. [0003] Dogs are important domestic animals for humans. They can not only be kept as pets, but also are...

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N15/877A01K67/027
CPCA01K67/0273A01K2227/10A01K2267/02C12N15/877
Inventor 郑敏米继东赵建平
Owner BEIJING SINOGENE BIOTECHNOLOGY CO LTD