Evaluation Method of Collagen Tissue on Gastric Serosa Surface in Resected Specimens of Gastric Cancer

An evaluation method and collagen tissue technology, applied in the field of pathological identification, can solve the problems of insufficient collagen information, limited accuracy, complicated and time-consuming operations, etc., and achieve good scientific research and promotion value, high resolution, and mature technology. Effect

Active Publication Date: 2020-08-21
NANFANG HOSPITAL OF SOUTHERN MEDICAL UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, there is no method for visually evaluating collagen on the surface of gastric serosa. The commonly used collagen analysis method—collagen (hydroxyproline) content analysis is time-consuming and has limited accuracy. Insufficient to provide adequate collagen information

Method used

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  • Evaluation Method of Collagen Tissue on Gastric Serosa Surface in Resected Specimens of Gastric Cancer
  • Evaluation Method of Collagen Tissue on Gastric Serosa Surface in Resected Specimens of Gastric Cancer
  • Evaluation Method of Collagen Tissue on Gastric Serosa Surface in Resected Specimens of Gastric Cancer

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Experimental program
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Effect test

Embodiment 1

[0055] Such as figure 1 Shown, a method for evaluating collagen tissue on the gastric serosa surface of a gastric cancer resection specimen, the method may further comprise the steps:

[0056] (a) Sample preparation: the gastric serosa surface tissue wax block was prepared after the gastric serosa tissue was separated from the gastric cancer resection specimen, and then a tissue section with a thickness of 3 μm was cut horizontally along the gastric serosa surface of the wax block, and the gastric subserosa Embedded facing downwards to prepare multiphoton imaging slices to be tested;

[0057] (b) Multiphoton collagen imaging: perform second harmonic imaging on the multiphoton imaging slice to be measured in step (a) to obtain a multiphoton collagen imaging image; the output power of the single-channel detector is 1.5W, and the imaging wavelength is 800nm , the receiving wavelength is 390nm, and the imaging multiple is 10 times;

[0058] (c) Section staining: perform Masson s...

Embodiment 2

[0061] A method for evaluating collagen tissue on the gastric serosa surface of a gastric cancer resection specimen, the method comprising the following steps:

[0062] (a) Sample preparation: the gastric serosa surface tissue wax block was prepared after the gastric serosa tissue was separated from the resection specimen of gastric cancer, and then a tissue section with a thickness of 8 μm was cut horizontally along the gastric serosa surface of the wax block, and the gastric subserosa Embedded facing downwards to prepare multiphoton imaging slices to be tested;

[0063] (b) Multiphoton collagen imaging: perform second harmonic imaging on the multiphoton imaging slice to be tested in step (a) to obtain a multiphoton collagen imaging image; the output power of the single-channel detector is 1.8W, and the imaging wavelength is 820nm , the receiving wavelength is 410nm, and the imaging multiple is 10 times;

[0064] (c) Section staining: perform Masson staining on the section a...

Embodiment 3

[0067] A method for evaluating collagen tissue on the gastric serosa surface of a gastric cancer resection specimen, the method comprising the following steps:

[0068] (a) Sample preparation: the gastric serosa surface tissue wax block was prepared after the gastric serosa tissue was separated from the resection specimen of gastric cancer, and then a tissue section with a thickness of 5 μm was cut horizontally along the gastric serosa surface of the wax block, and the gastric subserosa Embedded facing downwards to prepare multiphoton imaging slices to be tested;

[0069] (b) Multiphoton collagen imaging: perform second harmonic imaging on the multiphoton imaging slice to be tested in step (a) to obtain a multiphoton collagen imaging image; the output power of the single-channel detector is 1.6W, and the imaging wavelength is 810nm , the receiving wavelength is 400nm, and the imaging multiple is 10 times;

[0070] (c) Section staining: perform Masson staining on the section a...

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Abstract

The invention provides a method for the tissue evaluation of the gastric serosa surface collagens of a gastric carcinoma resection specimen, and relates to the technical field of pathological identification. The tissue evaluation of the gastric serosa surface collagens of the gastric carcinoma resection specimen is carried out by combing Masson staining with multiphoton imaging analysis. The method combining the two collagen analysis manners can rapidly, simply and accurately analyze the collagens on the surface of the gastric serosa, helps clinicians to know the status of the gastric serosa surface collagens of a patient, and has great reference values in the evaluation of the malignancy degree and the metastasis and recurrence possibility of tumors of the gastric carcinoma patient. The method has the advantages of reasonable design, mature technology, simplicity, easiness in implementation, broad clinical application prospect and good scientific research promotion values.

Description

technical field [0001] The invention relates to the technical field of pathological identification, in particular to a method for evaluating collagen tissue on the gastric serosa surface of a gastric cancer resection specimen. Background technique [0002] According to the latest cancer statistics in my country, gastric cancer is the second most common malignant tumor in my country and the second most lethal in cancer, and it is one of the major cancers threatening the health of Chinese residents. During the progression of gastric cancer, tumor cells break through from the mucosa to the submucosa and muscle layer layer by layer, and finally infiltrate the serosa layer and fall off to the peritoneal cavity to form implantation and metastasis. The serosal layer is the last barrier for tumor cells to enter the peritoneal cavity, so it is of great significance to understand the changes of the gastric serosal surface in gastric cancer for the study of peritoneal implantation and ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N21/64G01N21/84
CPCG01N21/6486G01N21/84
Inventor 严俊刘张苑珠李国新徐硕瑀许春辉陈德鑫蒋伟李凯陈伟生
Owner NANFANG HOSPITAL OF SOUTHERN MEDICAL UNIV
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