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Multidimensional microsphere coding and decoding method and system

A multi-dimensional, encoding and decoding technology, applied in the field of biochip detection, can solve the problem that the detection throughput cannot meet the clinical needs, and achieve the effect of automatic decoding, high throughput and efficiency improvement.

Inactive Publication Date: 2018-04-20
TSINGHUA BERKELEY SHENZHEN INST
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Problems solved by technology

[0005] Despite so many advantages, with the continuous emergence of new technologies and the further requirements of social development for detection technology, liquid-phase biochip technology still faces some difficulties: due to the increasing demand for detection indicators, the existing detection throughput It can no longer meet the clinical needs, so it is necessary to use a new codec method to improve the detection throughput

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  • Multidimensional microsphere coding and decoding method and system
  • Multidimensional microsphere coding and decoding method and system
  • Multidimensional microsphere coding and decoding method and system

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Embodiment 1

[0055] In this embodiment, the decoding method of the system is as follows Figure 4 shown, including the following steps:

[0056] S10, establishing a database 40. Prepare known coded fluorescent microspheres with different particle sizes, different fluorescence spectral characteristics or different fluorescent light intensities, and obtain the particle size data, fluorescent spectral waveforms, and fluorescent light intensity data of each fluorescent microsphere through instruments and equipment. The corresponding data is obtained by a statistical method as the data of the database 40, where the statistical method may be mean value, median value (including but not limited to) and the like. Specifically, the fluorescent microsphere code (the first code A and the second code B) is obtained through the data acquisition module 10 as multiple sets of known size data and fluorescent optical data, for example, M*N kinds of known codes are prepared (M represents The codable quanti...

Embodiment 2

[0084] In this embodiment, the system further includes a classifier, and the decoding method of the system is as follows: Image 6 shown, including the following steps:

[0085]S10', training a classifier, inputting the type of each fluorescent microsphere to be trained and the fluorescent optical data into the classifier to train the classifier to obtain a trained classifier.

[0086] The classifier is used to map the data to be tested to one of the given categories, so as to complete the classification of the data to be tested. The classifier includes support vector machine (SVM) and decision tree. Specifically in this embodiment, the classifier is used to map the fluorescent light intensity data of the fluorescent microsphere to be decoded to the type of the fluorescent microsphere to which it belongs, so as to realize the decoding of the second code of the fluorescent microsphere.

[0087] Generally speaking, in order to improve the performance of the classifier, the more...

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Abstract

The invention discloses a multidimensional microsphere coding and decoding method and system. The size and spectral signature of microspheres are taken as two dimensions and coded. The coding varietyis developed, and high-flux detection is achieved. Whether the size of a to-be-decoded microsphere belongs to a preset size range is decided, so that a first code used for identifying the size of microspheres is obtained. The optical data of to-be-decoded microspheres is classified via a classifier to obtain a second code, or according to the similarity of the optical data and known coded opticalreference data, a second code is obtained. Therefore, automatic decoding of two-dimensional coding is achieved, and the efficiency of biological detection is improved.

Description

technical field [0001] The invention relates to the technical field of biochip detection, in particular to a multi-dimensional microsphere encoding and decoding method and system. Background technique [0002] Liquid-phase biochip technology is a cutting-edge biomolecular detection technology integrating flow technology, fluorescent microsphere chemical synthesis technology, biomolecular hybridization technology, and high-efficiency digital signal processing technology. The core of liquid-phase biochip technology is functional polymer microspheres (fluorescent microspheres) with fluorescent coding labels, such as figure 1 Shown in a. The basic idea of ​​this technology is to immobilize specific probe molecules b on each encoded fluorescent microsphere a to obtain capture microspheres c and suspend them in a liquid phase system, using different encoded fluorescent substances (such as reporter molecules e ) After labeling the test sample d, put it into the above-mentioned li...

Claims

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Application Information

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IPC IPC(8): G01N33/543
CPCG01N33/54313
Inventor 马岚王东风
Owner TSINGHUA BERKELEY SHENZHEN INST
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