Endoglucanase and its coding gene cel5A-h28 and application

An endoglucanase and gene technology, applied in the field of genetic engineering, can solve problems such as slow process, achieve the effects of short production cycle, ensure product specificity, and ensure amplification efficiency
CN107964540AActive Publication Date: 2018-04-27ZHEJIANG UNIV
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
ZHEJIANG UNIV
Publication Date
2018-04-27

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Abstract

The invention belongs to the field of genetic engineering and particularly relates to an endoglucanase and its coding gene cel5A-h28 and application. The gene cel5A-h28 for encoding the endoglucanasehas a nucleotide sequence shown in the formula of SEQ ID No. 1. The bioinformatic analysis result shows that the endoglucanase coded by the gene belongs to the 5th family of glycoside hydrolase. Primers are designed and cloned and then are successfully expressed in a prokaryotic expression system. The recombinant endoglucanase obtained by induced ultrasonic purification has the optimal pH of 6.0 and the optimal temperature of 55 DEG C, keeps the stable enzyme activity at 40 DEG C, has strong pH tolerance and keeps the enzyme activity of 85% or more at pH of 4.0-9.0. The recombinant endoglucanase has the large research and industrial application potential.
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Description

technical field

[0001] The invention belongs to the field of genetic engineering, and specifically relates to an endoglucanase, its coding gene cel5A-h28 and application thereof. Background technique

[0002] As the main component of plant cell walls, cellulose is currently the most abundant renewable resource in nature, but its stubborn structure limits its utilization. The process of degrading cellulose into glucose requires endoglucanase (Endoglucanase, EC3.2.1.4), exoglucanase (exoglucanase, E.C.3.2.1.91) and β-glucosidase (β-glucosidase, E.C. 3.2.1.21) The three cellulase systems act together. Among them, endoglucanase is the most important component of the cellulase system, it mainly acts on the non-crystalline region of cellulose, randomly hydrolyzes the β-1,4 glycosidic bonds in the cellulose chain, cuts off the long chain of cellulose, It is converted into a large number of short chains with different degrees of polymerization, reducing the degree of polymerizatio...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
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