Tobacco CMV inoculating method

Abstract

The invention belongs to the technical field of inoculation of plant diseases and discloses a tobacco CMV inoculating method and system. The method comprises the following steps: collecting a typicaltobacco leaf of a CMV diseased plant for later use; when the tobacco plant inoculation amount is small, shearing a diseased leaf for 2-3 times by virtue of disinfected scissors, shearing a to-be-inoculated tobacco plant, and repeatedly carrying out the step until all to-be-inoculated tobacco plants are shorn; after tobacco leaves are shorn into pieces, collecting the pieces on clean paper, cloth or disc, and shearing the broken tobacco leaves for 2-3 times by virtue of the scissors, so as to shear the to-be-inoculated tobacco plants; and when the quantity of the to-be-inoculated tobacco plantsis large, preparing virus juice from standby diseased leaves, dipping the disinfected scissors into the virus juice once, shearing one to-be-inoculated tobacco plant, and repeatedly carrying out thestep until all to-be-inoculated tobacco plants are shorn. According to the method, the steps and the objects are simplified, and carborundum and gauzes are not used; and the technique of a friction inoculation method is very important, the back surfaces of the tobacco leaves are supported by one hand, the friction inoculation force on the other hand is very important, and the situations that the tobacco leaves are not damaged or are seriously damaged are improper.

Description

technical field [0001] The invention belongs to the technical field of plant disease inoculation, and in particular relates to a tobacco CMV inoculation method. Background technique [0002] Tobacco CMV inoculation is usually inoculated with virus juice friction. The collected CMV diseased leaves are made into virus inoculum, and sterilized 400-600 mesh emery is added to the virus inoculum. Take the virus juice and rub the leaves of the tobacco plant to be inoculated for inoculation. [0003] To sum up, the problem existing in the prior art is: the existing inoculation method causes insufficient or severe damage to the inoculated leaves, which easily leads to poor inoculation effect. In the prior art, the emery deposit added in the virus liquid requires constant stirring, which may easily cause uneven emery when dipping the inoculation juice. Existing inoculation methods are particularly difficult to operate when the inoculated leaves are smaller and tender. The existing ...

AI Technical Summary

Problems solved by technology

[0003] In summary, the existing problems in the prior art are: the existing inoculation methods cause insufficient or severe damage to the inoculated leaves, which may easily cause poor inoculation effects.

Existing inoculation methods tend to cause waste of virus juice, and the amount of virus juice required is relatively large, especially when the amount of inoculated tobacco plants is large, more typical CMV diseased leaves need to be taken to make more inoculum

Both emery and gauze used in the existing inoculation method need to be sterilized

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