COL1A1 (collagen type 1 alpha1) gene-containing detection kit for improving Altay sheep meat quality trait

A detection kit and a technology for meat quality traits, applied in the identification field of animal genetic selection and breeding, can solve the problems that there is no influence on the meat quality of Altay mutton, and the research and reports on the improvement of Altay sheep selection by COL1A1 gene have not been seen, and achieve good results. specific effect

Inactive Publication Date: 2018-05-04
XINJIANG AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The research on sheep is mainly aimed at the research of the disease prevention and control process. There is no research on the effect of this gene on the meat quality of Altay sheep, and the specific research on the improvement of the meat quality of the special Xinjiang typical breed of Altay sheep
In the prior art, there is no research and report on the use of COL1A1 gene to improve the meat quality traits of Altay mutton at home and abroad.

Method used

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  • COL1A1 (collagen type 1 alpha1) gene-containing detection kit for improving Altay sheep meat quality trait
  • COL1A1 (collagen type 1 alpha1) gene-containing detection kit for improving Altay sheep meat quality trait

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0032] The detection kit containing the COL1A1 gene to improve the quality traits of Altay mutton, the kit is specifically obtained through the establishment of the following co-construction system:

[0033] (1) Design primer pair: Use the mRNA sequence of sheep COL1A1 gene to design specific primers manually. The primers are as follows:

[0034] COL1A1 primers:

[0035] Upstream primer F: 5'-TACAGCGTCACCTACGACGG-3';

[0036] Downstream primer R: 5'-AAGCCGAATTCCTGGTCTG-3'.

[0037] (2) PCR reagents: Mix, upstream and downstream primers, cDNA template and RNase-free water.

Embodiment 2

[0039] The invention provides the application of a detection kit containing the COL1A1 gene to improve the quality traits of Altay mutton, comprising the following steps:

[0040] (1) Genomic RNA extraction:

[0041] Collect 30-50 mg longissimus dorsi muscle of the Altay sheep to be tested, store in liquid nitrogen; extract RNA, store at -80°C; reverse transcribe into cDNA, and perform PCR amplification with the internal reference gene ACTB.

[0042] Prepare 1.5% agarose gel: Dissolve 1.5g agarose in 100ml 0.5×TBE, heat it in a microwave until fully dissolved, pour it into a gel tank with a comb inserted after cooling down to room temperature, cool and solidify before use.

[0043] The prepared 1.5% agarose gel was used for electrophoresis detection, the voltage was 100V, the current was 50mA, and the electrophoresis was performed for 35 minutes. After staining with ammonium bromide for 15 minutes, irradiate under the ultraviolet light of a gel imager, observe the size and br...

Embodiment 3

[0057] Based on Example 2, the detection kits containing the COL1A1 gene provided in the above examples to improve the meat quality traits of Altay mutton were applied to pigs, Octave Brook sheep, Taxkorgan sheep, Bashbai sheep, Xinjiang fine-wool sheep, Kirgiz sheep, and Hetian sheep. In the longissimus dorsi, the relationship between the expression of COL1A1 gene in the longissimus dorsi and the intramuscular fatty acid content, water content and shear force of the longissimus dorsi was observed. See Table 2 for details:

[0058] Table 2: Correlation analysis between COL1A1 gene expression and some meat quality indicators

[0059]

fatty acid content

moisture content

longissimus dorsi shear force

altay sheep

1.000*

-0.997*

0.99 *

pig

0.192

0.120

0.162

tone brook sheep

0.141

0.041

0.106

Taxkorgan sheep

0.157

0.113

0.105

Bashbai sheep

0.121

0.085

0.046

Xinjiang fine wool...

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Abstract

The invention discloses a COL1A1 (collagen type 1 alpha1) gene-containing detection kit for improving Altay sheep meat quality trait. A specific primer is self and artificially designed according to an mRNA sequence of a sheep COL1A1 gene, an upstream primer is 5'-TACAGCGTCACCTACGACGG-3', a downstream primer is 5'-AAGCCGAATTCCTGGTCTG-3', sample genome DNA is utilized as a template to perform PCR proliferation, fluorescence quantitative PCR detection is performed on a PCR proliferation product, correlation analysis between gene expression quantity and intramuscular fatty acid content, moisturecontent and longissimus dorsi shear force is performed, and the COL1A1 gene-containing detection kit disclosed by the invention is shown to have better specificity to the Altay sheep. The COL1A1 gene-containing detection kit disclosed by the invention not only fills the blank of the research field at home and abroad, but also has important significance and actual application value in protecting Altay sheep good stock resource development, establishing molecular theory basis for sustainable utilization and quickening Sinkiang mutton sheep industry development.

Description

technical field [0001] The invention relates to an identification method for animal genetic selection and breeding, specifically, the invention relates to the technical field of a kit for selection of meat quality traits of Altay mutton. Background technique [0002] Xinjiang is one of the five major pastoral areas in my country, and the total output of mutton ranks among the top in the country. At the same time, as a minority area in Xinjiang, the demand for mutton is growing rigidly, making it difficult for the mutton production in the region to meet the growing demand of people. To solve this problem, it is particularly important to analyze the genetic mechanism attached to the abundant and unique sheep breeds in Xinjiang. Altay sheep has a long history and is commonly known as Fuhai big-tailed sheep. It is the main breed in the animal husbandry structure in Xinjiang, and has obtained geographical indications, which have been included in the "China Geographical Indication...

Claims

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Application Information

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IPC IPC(8): C12Q1/6888
CPCC12Q1/6888C12Q2600/124C12Q2600/158C12Q2600/166
Inventor 刘武军赵国艺方超马海玉方丽君刘玲玲于茜褚洪忠
Owner XINJIANG AGRI UNIV
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