Detection method of grub

Abstract

The invention discloses a detection method of holotrichia diomphalia bates. The detection method comprises thin-layer chromatographic identification and determination of inosine and guanosine in the holotrichia diomphalia bates, wherein the thin-layer chromatographic identification comprises the following specific operation steps: preparing a test solution and a reference medicine solution, testing by referring to thin-layer chromatography, developing by using a mixture of cyclohexane, ethyl acetate and methanol as a developing agent according to a ratio of 10 to 10 to 0.5, and inspecting under an ultraviolet lamp; the determination of the inosine and the guanosine in the holotrichia diomphalia bates comprises the following specific operation steps: preparing a test solution and a mixed reference medicine solution, and detecting under the following chromatographic conditions: with acetonitrile as a mobile phase A and trifluoroacetic acid with the concentration of 0.08% as a mobile phase B, gradient elution is performed, the detection wavelength is 260nm, the flow rate is 1.0 mL / min, an ultraviolet detector is used, the column temperature is 30 DEG C, and the feeding volume is 10 microliters. By the detection method, the quality of the holotrichia diomphalia bates can be effectively evaluated; the detection method is simple, fast, accurate and good in reproducibility.

Description

technical field [0001] The invention belongs to the field of detection of traditional Chinese medicinal materials, and in particular relates to a detection method of grubs. Background technique [0002] Grubs are the dried larvae of Holotrichia diomphalia Bates, an insect of Scarabidae. It is recorded in the second volume of "Dictionary of Chinese Medicine", page 3224 of the second edition, and is a traditional medicinal material in my country. Grub has the effects of breaking blood stasis, dispelling stagnation, relieving pain, detoxifying, etc.; it is mainly used for blood stasis amenorrhea, lumps in the abdomen, wounds and bruises, gout, tetanus, sore throat, carbuncle, erysipelas, etc. [0003] In most literature reports, the quality of grubs is evaluated by amino acid determination and nitrogen determination of protein, but there is no specific research on the quality control of grubs. rhubarb Chongwan (recorded in "Chinese Pharmacopoeia" 2015 edition one 499 pages)...

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Problems solved by technology

[0003] In most of the literature reports, the quality of grubs is evaluated by methods such as amino acid determination and nitrogen determination of protein, but there is no specific research on the quality control of grubs

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