Plants with increased seed size

A technology of plants and homologues, applied in the direction of angiosperms/flowering plants, plant peptides, plant products, etc., can solve the problem of increasing cell proliferation

Inactive Publication Date: 2018-05-08
INST OF GENETICS & DEVELOPMENTAL BIOLOGY CHINESE ACAD OF SCI
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  • Summary
  • Abstract
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  • Application Information

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Problems solved by technology

In contrast, overexpression of CYP78A6 / EOD3 increased cell proliferation and cell elongation in the integument, resulting in larger seeds (Fang et al., 2012)

Method used

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  • Plants with increased seed size
  • Plants with increased seed size
  • Plants with increased seed size

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Experimental program
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Embodiment

[0163] method

[0164] Plant material and growing conditions

[0165] Arabidopsis Columbia (Col-0) was used as the wild-type line. da1-1, sod7-1D, sod7-ko1 and ngal3-ko1 in the Col-0 background. sod7-1D was identified as a dal-1 repressor by using the T-DNA activation tagging approach. sod7-ko1(SM_3_34191) and ngal3-ko1(SM_3_36641) were identified in AtIDB ( www.atidb.org ) and from the ArabidopsisStock Center NASC Collection. T-DNA insertion was confirmed by PCR, and by sequencing using the primers shown in Table 1. Arabidopsis plants were grown at 22°C under long day conditions (16 hours light / 8 hours dark). Activate tag screening. The activation-tagged plasmid pJFAT260 was introduced into da1-1 mutant plants using Agrobacterium tumefaciens strain GV3101 (Fan et al., 2009; Fang et al., 2012), and T1 plants were selected using the herbicide Basta. Seeds produced from T1 plants were used to isolate modifiers of dal-1.

[0166] Morphological and cellular analysis ...

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Abstract

The invention relates to genetically modified plants with an altered seed phenotype, in particular increased seed size. The invention relates to a plant that does not produce a functional NGAL2 polypeptide or functional NGAL2 and NGAL3 polypeptides. NGAL2 and NGAL3 are members of the RAV family and comprise a B3 DNA- binding domain and a transcriptional repression motif.

Description

field of invention [0001] The present invention relates to transgenic plants having improved growth and yield-related traits, in particular increased seed size. The invention also relates to related methods, uses, isolated nucleic acids and vector constructs. Background of the invention [0002] The ever-increasing world population and decreasing supply of arable land available for agriculture requires research on increasing the efficiency of agronomy and providing food security. A conventional approach to crop and horticultural improvement is to use selective breeding techniques to identify plants with desirable traits. However, such selective breeding techniques have some disadvantages, namely that these techniques are typically laborious and result in plants that often contain heterologous genetic components that may not always result in the desired trait when passed from the parent plant. Advances in molecular biology have allowed humans to modify the germplasm of anim...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H5/10A01H5/00A01H6/46C12N15/29C12N15/82
CPCA01H5/10C07K14/415C12N15/8218C12N15/8261Y02A40/146
Inventor 李云海
Owner INST OF GENETICS & DEVELOPMENTAL BIOLOGY CHINESE ACAD OF SCI
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