Tissue culture medium of litsea cubeba

A technology of tissue culture and culture medium, applied in horticulture, botany equipment and methods, gardening tools/equipment, etc., can solve the problems of low effective seedling rate, low overall efficiency of tissue culture, high incidence of browning of tissue culture seedlings, etc. To achieve the effect of improving the composition and content

Inactive Publication Date: 2018-05-11
FUJIAN ACAD OF FORESTRY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

We adopt existing technical methods and find in research and production practice that there are still problems such as high incidence of browning of tissue cultured seedlings, weak growth of tissue cultured aseptic sprouts, and low rate of effective seedlings, and the overall efficiency of tissue culture is low, making tissue culture Cultivation technology is difficult to apply on a large scale in production

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0020] The components and the content of each component of 1L strong seedling medium are as follows, the unit is mg:

[0021] (1) Macroelement: 1100 ammonium nitrate NH 4 NO 3 , 1267 potassium nitrate KNO 3 , 136 potassium dihydrogen phosphate KH 2 PO 4 , 264 magnesium sulfate heptahydrate MgSO 4 .7H 2 O, 385 Calcium Chloride Anhydrous CaCl 2 ;

[0022] (2) Trace elements: 0.42 potassium iodide KI, 17.84 manganese sulfate MnSO 4 .H 2 O, 4.96 boric acid H 3 BO 3 , 7.5 Zinc Sulfate ZnSO 4 .7H 2 O, 0.25 sodium molybdate Na 2 MoO 4 .2H 2 O, 0.025 copper sulfate CuSO 4 .5H 2 O,0.025 cobalt chloride CoCl 2 .6H 2 O;

[0023] (3) Iron salt: 33.36 ferrous sulfate FeSO 4 .7H 2 O, 44.76 EDTA disodium Na 2 .EDTA;

[0024] (4) Organic add-on: 100 Inositol C 6 h 12 o 6 , 0.5 Niacin C 6 h 5 NO 2 , 0.125 Thiamine Hydrochloride C 12 h 17 ClN 4 OS·HCl, 0.83 dopyridine hydrochloride C 8 h 11 NO 3 • HCl, 2 Glycine C 2 h 5 NO 2 ;

[0025] (5) Plant growth re...

Embodiment 2

[0036] The components and the content of each component of 1L strong seedling medium are as follows:

[0037] (1) Macroelement: 1100 ammonium nitrate NH 4 NO 3 , 1267 potassium nitrate KNO 3 , 136 potassium dihydrogen phosphate KH 2 PO 4 , 264 magnesium sulfate heptahydrate MgSO 4 .7H 2 O, 385 Calcium Chloride Anhydrous CaCl 2 ;

[0038] (2) Trace elements: 0.42 potassium iodide KI, 17.84 manganese sulfate MnSO 4 .H 2 O, 4.96 boric acid H 3 BO 3 , 7.5 Zinc Sulfate ZnSO 4 .7H 2 O, 0.25 sodium molybdate Na 2 MoO 4 .2H 2 O, 0.025 copper sulfate CuSO 4 .5H 2 O, 0.025 cobalt chloride CoCl 2 .6H 2 O;

[0039] (3) Iron salt: 33.36 ferrous sulfate FeSO 4 .7H 2 O, 44.76 EDTA disodium Na 2 .EDTA;

[0040] (4) Organic add-on: 100 Inositol C 6 h 12 o 6 , 0.5 Niacin C 6 h 5 NO 2 , 0.125 Thiamine Hydrochloride C 12 h 17 ClN 4 OS·HCl, 0.83 dopyridine hydrochloride C 8 h 11 NO 3 • HCl, 2 Glycine C 2 h 5 NO 2 ;

[0041] (5) Plant growth regulators and ot...

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PUM

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Abstract

The invention relates to a tissue culture medium of litsea cubeba. The tissue culture medium is prepared from a sterile culture sound seedling culture medium and a rooting culture medium; on the basisof an MS culture medium, rare-earth elements are added and the dosage of nutrient elements is systematically improved; the culture medium provided by the invention is more suitable for growth of litsea cubeba tissue culture test-tube plantlets. By adopting the tissue culture medium provided by the invention, the problems including that browning easily occurs in litsea cubeba tissue culture and the test-tube plantlets are thin and weak in the prior art, which are not good for proliferation of tissue culture seedlings and transplanting survival rate, are solved.

Description

technical field [0001] The invention belongs to the technical field of rapid propagation of plant tissue culture, and in particular relates to a tissue culture medium of litsea cubeba. Background technique [0002] Litsea Cubeba is a precious aromatic oil-bearing small tree belonging to the family Zingiberaceae. Its flowers, leaves, fruits and bark can be distilled to extract Litsea cubeba oil, and the mature fruit has the highest oil content. Litsea cubeba oil contains citral, higher alcohols, and organic acids. The citral content is as high as 70%. It is the main raw material for preparing flavors, fragrances, and synthetic vitamins A and E. It can also be directly used for insecticide and sterilization. The largest natural plant essential oils. Litsea Cubeba is mainly distributed in Zhejiang, Fujian, Hunan and other provinces in my country, and it is a tree species with highly unified ecological, economic and social benefits. Traditional propagation mainly uses seeds to...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00
CPCA01H4/001
Inventor 范辉华王志洁张娟汤行昊黄宇蒋泽平
Owner FUJIAN ACAD OF FORESTRY
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