Tissue culture medium of litsea cubeba
A technology of tissue culture and culture medium, applied in horticulture, botany equipment and methods, gardening tools/equipment, etc., can solve the problems of low effective seedling rate, low overall efficiency of tissue culture, high incidence of browning of tissue culture seedlings, etc. To achieve the effect of improving the composition and content
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Embodiment 1
[0020] The components and the content of each component of 1L strong seedling medium are as follows, the unit is mg:
[0021] (1) Macroelement: 1100 ammonium nitrate NH 4 NO 3 , 1267 potassium nitrate KNO 3 , 136 potassium dihydrogen phosphate KH 2 PO 4 , 264 magnesium sulfate heptahydrate MgSO 4 .7H 2 O, 385 Calcium Chloride Anhydrous CaCl 2 ;
[0022] (2) Trace elements: 0.42 potassium iodide KI, 17.84 manganese sulfate MnSO 4 .H 2 O, 4.96 boric acid H 3 BO 3 , 7.5 Zinc Sulfate ZnSO 4 .7H 2 O, 0.25 sodium molybdate Na 2 MoO 4 .2H 2 O, 0.025 copper sulfate CuSO 4 .5H 2 O,0.025 cobalt chloride CoCl 2 .6H 2 O;
[0023] (3) Iron salt: 33.36 ferrous sulfate FeSO 4 .7H 2 O, 44.76 EDTA disodium Na 2 .EDTA;
[0024] (4) Organic add-on: 100 Inositol C 6 h 12 o 6 , 0.5 Niacin C 6 h 5 NO 2 , 0.125 Thiamine Hydrochloride C 12 h 17 ClN 4 OS·HCl, 0.83 dopyridine hydrochloride C 8 h 11 NO 3 • HCl, 2 Glycine C 2 h 5 NO 2 ;
[0025] (5) Plant growth re...
Embodiment 2
[0036] The components and the content of each component of 1L strong seedling medium are as follows:
[0037] (1) Macroelement: 1100 ammonium nitrate NH 4 NO 3 , 1267 potassium nitrate KNO 3 , 136 potassium dihydrogen phosphate KH 2 PO 4 , 264 magnesium sulfate heptahydrate MgSO 4 .7H 2 O, 385 Calcium Chloride Anhydrous CaCl 2 ;
[0038] (2) Trace elements: 0.42 potassium iodide KI, 17.84 manganese sulfate MnSO 4 .H 2 O, 4.96 boric acid H 3 BO 3 , 7.5 Zinc Sulfate ZnSO 4 .7H 2 O, 0.25 sodium molybdate Na 2 MoO 4 .2H 2 O, 0.025 copper sulfate CuSO 4 .5H 2 O, 0.025 cobalt chloride CoCl 2 .6H 2 O;
[0039] (3) Iron salt: 33.36 ferrous sulfate FeSO 4 .7H 2 O, 44.76 EDTA disodium Na 2 .EDTA;
[0040] (4) Organic add-on: 100 Inositol C 6 h 12 o 6 , 0.5 Niacin C 6 h 5 NO 2 , 0.125 Thiamine Hydrochloride C 12 h 17 ClN 4 OS·HCl, 0.83 dopyridine hydrochloride C 8 h 11 NO 3 • HCl, 2 Glycine C 2 h 5 NO 2 ;
[0041] (5) Plant growth regulators and ot...
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