Unlock instant, AI-driven research and patent intelligence for your innovation.

An ecological biomimetic microbial flocculant based on Philippine clam adhesion sludge and its preparation method

A microbial flocculant and Philippine clam technology, applied in the field of water treatment, can solve the problems of new pollution, secondary pollution, and consumption of organic solvents in the treatment system, and achieve the removal of pollutants and suspended particles, good bio-sedimentation, and Good flocculation effect

Active Publication Date: 2020-09-18
ZHEJIANG OCEAN UNIV
View PDF4 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The disadvantage of fermented liquid containing bacteria is that directly adding fermented liquid when treating wastewater will artificially add new pollution to the treatment system, waste culture medium and reduce treatment efficiency; In addition to the production cost, secondary pollution, substrate waste and low treatment efficiency still exist; the preparation of polysaccharide complexes requires centrifugation and consumes a large amount of organic solvents, the cost is even higher, and the prospect of large-scale industrial development for water treatment is difficult to appear

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Examples

Experimental program
Comparison scheme
Effect test

Embodiment approach

[0025] An ecological biomimetic microbial flocculant based on the adherent sludge of Philippine clams, which is cultured by inoculating the sludge suspension of Philippine clams into liquid culture medium, and then naturally settling or centrifuging to separate the sediment and freeze-drying Obtained afterwards; the formula of the liquid medium is: glucose 10-25g / L, urea 0.2-0.8g / L, ammonium sulfate 0.1-0.5g / L, yeast extract 0.2-0.8g / L, dipotassium hydrogen phosphate 1.25-5g / L, potassium dihydrogen phosphate 0.5-2g / L, carrier 50-90g / L, artificial seawater balance, adjust pH to 7.2-8. The carrier is one or more of sand, silt, sea sand, diatomaceous earth, active carbon and shell powder.

[0026] Artificial Seawater Formula:

[0027] Sodium chloride NaCl 25.0 g / L

[0028] Magnesium Chloride MgCl 2 2. 0 g / L

[0029] Magnesium Sulfate MgSO 4 3.2g / L

[0030] Calcium Chloride CaCl 2 1.2g / L

[0031] Sodium bicarbonate NaHCO 3 0.2 g / L

[0032] Potassium chloride KCl 0.7 g...

Embodiment 1

[0044] A preparation method of an ecological biomimetic microbial flocculant based on philippine clam adhesion sludge, comprising the steps of:

[0045] (1) Take five native unwashed Philippine clams (about 7g / head) and wash them in conical flasks filled with 100mL of sterile seawater, wash them three times in turn, and put them directly in the third culture bottle. Put and spit out mud for 24 hours, thereby obtain the sludge suspension liquid of Filipino clam spit;

[0046] (2) Prepare culture medium with artificial seawater in advance: glucose 20g / L, urea 0.5g / L, ammonium sulfate 0.2g / L, yeast extract 0.5g / L, dipotassium hydrogen phosphate (K 2 HPO 4 ) 5g / L, potassium dihydrogen phosphate (KH 2 PO 4 ) 2g / L, the amount of soil (sand) added is 70

[0047] The sandy soil is prepared according to the weight ratio of 70% sea sand and 30% soil.

[0048] (3) Inoculate 0.5mL of the sludge suspension of Philippine clams into the above 100mL culture medium;

[0049] (4) Place th...

Embodiment 2

[0052] A preparation method of an ecological biomimetic microbial flocculant based on philippine clam adhesive sludge, comprising the steps of:

[0053] (1) Take five native unwashed Philippine clams (about 7g / head) and wash them in conical flasks filled with 100mL of sterile seawater, wash them three times in turn, and put them directly in the third culture bottle. Put and spit out mud for 24 hours, thereby obtain the sludge suspension liquid of Filipino clam spit;

[0054] (2) Prepare medium with old sea water in advance: glucose 15g / L, urea 0.5g / L, ammonium sulfate 0.2g / L, yeast extract 0.5g / L, dipotassium hydrogen phosphate (K 2 HPO 4 ) 5g / L, potassium dihydrogen phosphate (KH 2 PO 4 ) 2g /

[0055] (3) Inoculate 0.5mL of the sludge suspension of Philippine clams into the above 100mL culture medium;

[0056] (4) Place the inoculated culture bottle in a shaker incubator with a temperature of 25°C and a rotation speed of 180r / min for 36 hours;

[0057] (5) After 36 hour...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention discloses an ecological bionic type microbial flocculant based on Ruditapes philippinarum conglutination sludge and a preparation method thereof. The ecological bionic type microbial flocculant is obtained as follows: after mud suspension vomited by Ruditapes philippinarum conglutination sludge is inoculated into a liquid culture medium for culturing, a precipitate is obtained by natural sedimentation or centrifugal separation, and the precipitate is freeze-dried to obtain the ecological bionic type microbial flocculant; the liquid culture medium is formulated as follows: 10-25 g / L of glucose, 0.2-0.8 g / L of urea, 0.1-0.5 g / L of ammonium sulfate, 0.2-0.8g / L of yeast extract, 1.25-5g / L of dipotassium hydrogen phosphate, 0.5-2g / L of potassium dihydrogen phosphate, 50-90g / L of acarrier, and balance of artificial seawater. The pH is adjusted to 7.2-8. The flocculant has a scientific and simple formulation ratio and good biological sedimentation property, and the flocculationrate can reach 73.62%.

Description

technical field [0001] The invention relates to the technical field of water treatment, in particular to an ecological bionic microbial flocculant based on philippine clam adhesion sludge and a preparation method thereof. Background technique [0002] Flocculants are widely and critically used in water treatment, sewage purification, food and fermentation engineering and other fields. According to the molecular composition, flocculants can be divided into three categories: inorganic flocculants, organic synthetic polymer flocculants and natural polymer flocculants. Inorganic flocculants are cheap, but will have adverse effects on human health and the ecological environment; organic synthetic polymer flocculants such as polyacrylamide, its monomer acrylamide has strong carcinogenicity and neurotoxicity to humans; polyacrylamide is also difficult to degrade , easy to accumulate in the environment and cause secondary pollution. The preparation of natural polymer flocculants f...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Patents(China)
IPC IPC(8): C02F3/34
CPCC02F3/34C02F2209/10C02F2305/06
Inventor 穆军王东方穆思源崔霞
Owner ZHEJIANG OCEAN UNIV