One-step embryo culture solution and preparation method thereof

Abstract

The invention belongs to the field of assisted reproduction, and particularly relates to a one-step embryo culture solution and a preparation method thereof. A composition for preparing the one-step embryo culture solution is prepared from (+ / -)-alpha-lipoic acid, acetyl L-carnitine or acetyl L-carnitine hydrochloride, wherein a molar ratio of the (+ / -)-alpha-lipoic acid to the acetyl L-carnitineor the acetyl L-carnitine hydrochloride is (0.055 to 0.098) : (0.044 to 0.078). The one-step embryo culture solution prepared by utilizing the composition has the advantages that effective componentssecreted into the culture solution by embryo are retained, the effects of protecting cells, inhibiting the decomposition and utilizing harmful products are achieved, and possible mechanical damage inthe process of embryo transfer is avoided; in addition, the problem of low formation rate of blastocyst caused by stress response due to different culture environments is solved.

Description

Technical field [0001] The invention belongs to the field of assisted reproduction, and specifically relates to a one-step embryo culture solution and a preparation method thereof. Background technique [0002] InVitro Fertilization-Embryo Transfer, referred to as IVF-ET, refers to the in vitro fertilization after the egg and sperm are taken out respectively, and then the embryo precursor-the fertilized egg is transferred back to the mother's uterus to develop into a fetus. Test-tube babies are babies born by artificially fertilizing eggs and sperm in vitro and carrying out early embryo development, and then transplanting them into the mother's womb. [0003] Since the world's first "test-tube" baby was born in the UK on July 25, 1978, 5 million "test-tube" babies have been born on July 1, 2012. IVF has brought good news to more than 50 million infertile couples worldwide. However, more than 30 years of clinical practice has not significantly improved the success rate of IVF. In...

AI Technical Summary

Problems solved by technology

[0005] The nutrition of the currently used semen preparation is incomplete, or its physical or chemical indicators are too wide, the same test process, the same batch of semen may easily cause the death of sperm or egg cells during the culture process, or the sperm capacitation is insufficient and the motility is low, or Egg viability is compromised, as in a sperm motility test, with a low sperm viability or a rate close to or below the qualifying criteria (≥75% viability)

Some fertilized semen have a high consistency, which contains harmful ingredients and inflammatory cells are not removed cleanly, which seriously affects the development and growth of fertilized eggs

[0006] In addition, the effectiveness of the general culture medium cannot be visually judged before use. If it continues to be used after it has deteriorated, it will cause irreversible results to the test or clinical operation.

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