Amino acid sequence, nucleic acid sequence and application of nucleic acid sequence

A nucleic acid sequence and amino acid technology, applied in the field of amino acid sequence and nucleic acid sequence, can solve problems such as drug resistance, water pollution, and pest production

Active Publication Date: 2018-05-18
YUNNAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, chemical pesticides are mainly used for prevention and control. However, due to the large amount and long-term use of chemical pesticides, on the one han...

Method used

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  • Amino acid sequence, nucleic acid sequence and application of nucleic acid sequence
  • Amino acid sequence, nucleic acid sequence and application of nucleic acid sequence
  • Amino acid sequence, nucleic acid sequence and application of nucleic acid sequence

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0037] 1. Design of Dip3-specific primers

[0038] Transcriptome data from Spodoptera litura After Parasitization by Microplitis bicoloratus.PLoS ONE 9( 10):e110967.doi:10.1371 / journal.pone.0110967), the Dip3 gene sequence was selected, the size of the Dip3 gene sequence (as shown in SEQ ID No.2) was 750bp, and it encoded a total of 249 amino acids (SEQ ID No.1). The primer design software Primer Premier 5 was used to design specific primers: upstream primer pIZT / V5-His-Dip3-F (SEQ ID No.3) and downstream primer pIZT / V5-His-Dip3-R (SEQ ID No.4).

[0039] 2. Extraction of total RNA and cDNA synthesis of Spodoptera litura larvae

[0040] In this experiment, the laboratory protocol was used to extract total RNA from Spodoptera litura larvae. The specific steps are as follows:

[0041] 1) Take 10 3-day-old larvae, place them in EP tubes with a small amount of RNAisoTM Plus lysate, mash and grind them thoroughly, continue to add RNAisoTM Plus lysate until the total volume reaches...

Embodiment 2

[0082] 1. Vank86, Vank92, Vank101 specific primer design

[0083] A polydnaviral genome of microplitis bicoloratus bracovirus (Yu, D.S., Chen, Y.B., Li, M., Yang, M.J., Yang, Y., & Hu, J.S., et al. (2016). A polydnaviral genome of microplitis bicoloratus bracovirus and molecular interactions between the host and virus involved innf-κb signaling.Archives of Virology, 161 (11), 3095.) select Vank86, Vank92 and Vank101 gene sequences, Vank86 gene sequence (as shown in SEQ ID No.9) size It is 516bp, encoding a total of 171 amino acids (SEQ ID No.10); the Vank92 gene sequence (as shown in SEQ ID No.11) is 501bp in size, encoding a total of 166 amino acids (SEQ ID No.12); the Vank101 gene sequence ( SEQ ID No.13) has a size of 558bp and encodes a total of 186 amino acids (SEQ ID No.14). Using primer design software Primer Premier 5 to design specific primers, upstream primer Vank86-F (SEQ ID No.15, containing EcoR I restriction site) and downstream primer Vank86-R (SEQ ID No.16, co...

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PUM

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Abstract

The invention relates to a nucleic acid sequence capable of being used for molecular control of pests and an application of the nucleic acid sequence. The nucleic acid sequence is as shown in SEQ ID No. 1 and can be used for controlling Spodoptera litura Fabricius.

Description

technical field [0001] The present application relates to an amino acid sequence and a nucleic acid sequence, in particular to the use of the amino acid sequence and / or nucleic acid sequence in identifying whether a gene in the pest genome of the genus Spodoptera is its dependent type and / or controlling the genus Spodoptera (Spodoptera) pest application. Background technique [0002] Spodoptera litura Fabricius (Spodoptera litura Fabricius), commonly known as night robber, night tiger, dew insect, taro insect, etc., belongs to the Lepidoptera family Noctuidae. It is widely distributed all over the world and occurs in all parts of my country. It is a typical omnivorous insect. It is known that there are more than 290 kinds of plants in 99 families, including crops such as cotton, tobacco and vegetables. Because Spodoptera litura has strong reproductive ability and is easy to develop resistance to pesticides, in recent years, the damage to vegetables and flowers has become mo...

Claims

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Application Information

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IPC IPC(8): C12N15/12C07K14/435C12Q1/6888A01N43/08A01N37/46A01P7/04
CPCA01N37/46A01N43/08C07K14/43563C12Q1/6888
Inventor 罗开珺张玮李明蔡秋宸徐沙崔继辉张雪雯张力丹何浩娟寇田超董树美
Owner YUNNAN UNIV
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