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Qaidam agaricus bitorquis anti-hypoxia polysaccharide identification method

An identification method, the technology of big fat mushrooms, is applied in the direction of material analysis through observation of the influence of chemical indicators, analysis by nuclear magnetic resonance, instruments, etc., and can solve problems such as functional application constraints

Inactive Publication Date: 2018-05-18
QINGHAI UNIVERSITY
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Problems solved by technology

Due to the fact that many parameters of the anti-hypoxic polysaccharide of Qaidam big fat mushroom have not obtained effective data, the functional application of Qaidam big fat mushroom anti-hypoxic polysaccharide has been restricted

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  • Qaidam agaricus bitorquis anti-hypoxia polysaccharide identification method
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  • Qaidam agaricus bitorquis anti-hypoxia polysaccharide identification method

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Embodiment Construction

[0039] The present invention will be further described below in conjunction with specific examples, but this does not imply any limitation to the present invention.

[0040] The identification method of the anti-hypoxic polysaccharide of Qaidam big fat mushroom is carried out according to the following steps:

[0041] 1. Determination of the molecular weight range of polysaccharides in Qaidam big fat mushroom

[0042] Determination process of polysaccharide molecular weight: drawing molecular weight standard curve according to standard dextran→determination of sample molecular weight→determination of molecular weight;

[0043] First measure the retention time of the dextran standard substance in the DEAE-52 gel column, take the retention time tR of the known molecular weight standard substance as the abscissa, and take the logarithm of the corresponding molecular weight as the ordinate lgM N , draw the calibration curve of the molecular weight of the standard substance;

[0...

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Abstract

The invention discloses a Qaidam agaricus bitorquis anti-hypoxia polysaccharide identification method which comprises the following steps of performing molecular weight determination, polysaccharide content determination, structural identification and anti-hypoxia functional characteristic judgment on polysaccharides in three sources to acquire various parameters such as the Qaidam agaricus bitorquis anti-hypoxia polysaccharide molecular weight, the polysaccharide content, the polysaccharide structural characteristics, the anti-hypoxia functional characteristic and the like, and therefore, theidentification method provides data support for actual application of Qaidam agaricus bitorquis anti-hypoxia polysaccharide, enables the efficacy of the Qaidam agaricus bitorquis anti-hypoxia polysaccharide to be exerted and provides help for healthy development of humans.

Description

technical field [0001] The invention relates to the technical field of biological detection, in particular to an identification and detection method for the anti-hypoxic polysaccharide of Qaidam big fat mushroom. Background technique [0002] Qaidam big fat mushroom Agaricus bitorquis (QuéL.) Sacc. Also known as double-layered ring mushroom, it belongs to the species of Agaricus bisporus in classification. It is a kind of wild large edible fungus with very important classification under the special ecological environment conditions of the Qaidam Basin on the Qinghai-Tibet Plateau. . Because it grows in a harsh environment, it has the characteristics of huge fruiting bodies, strong stress resistance, firm underground, and low-temperature fruiting. It is a special strain of big fat mushrooms. The fruit body of Qaidam big fat mushroom contains 24.0% crude protein, 2.10% crude fat, 17.32% crude fiber, 34.91% carbohydrate and 13.65% ash. Since the Qaidam big fat mushroom is a s...

Claims

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Application Information

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IPC IPC(8): G01N21/78G01N21/31G01N24/08
CPCG01N21/31G01N21/78G01N24/087
Inventor 焦迎春刘文卓吴杉
Owner QINGHAI UNIVERSITY
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