Fertilization-reducing and synergism-improving method of Vicia sativa L. inoculated with rhizobium in tobacco land crop rotation

A technology of rhizobia and rhizobia is applied in the field of reducing application and increasing efficiency of rhizobia-inoculated rhizobia in the field of tobacco field rotation, which can solve the problems of few reports of leguminous green manure inoculating rhizobia, reduce the use of chemical fertilizers, improve the Tobacco yield, the effect of reducing soil pollution

Inactive Publication Date: 2018-06-08
SICHUAN AGRI UNIV
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AI Technical Summary

Problems solved by technology

In the production process of legume green manure, only rhizobia are inoculated, and no fertilize

Method used

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  • Fertilization-reducing and synergism-improving method of Vicia sativa L. inoculated with rhizobium in tobacco land crop rotation
  • Fertilization-reducing and synergism-improving method of Vicia sativa L. inoculated with rhizobium in tobacco land crop rotation
  • Fertilization-reducing and synergism-improving method of Vicia sativa L. inoculated with rhizobium in tobacco land crop rotation

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0031] Embodiment 1 prepares rhizobia bacterial agent

[0032] (1) Isolation and purification of Rhizobium japonica JSP1-3-1

[0033] Collect the root nodules of wild and strong arrowhead peas in Jianzhu Township, Gulin, Luzhou, Sichuan Province, clean them, take off part of the root bark, absorb the surface moisture with absorbent paper, and store them in a box with anhydrous calcium chloride and covered with absorbent cotton in a small tube. Carry out the following operations in the laboratory: the root nodules collected are soaked in sterile water for imbibition, soaked in 95% ethanol for 30s to eliminate surface tension, then sterilized with 0.1% (mass volume ratio) mercury chloride surface for 5min, and then use sterile Rinse with bacterium water 6-8 times, under the situation of aseptic operation, clamp the single root nodule and add Congo red YMA medium (mannitol 10g, yeast powder 0.8g, KH 2 PO 4 0.25g, MgSO 4 .7H 2 O 0.2g, CaCl 2 .6H 2 O 0.1g, NaCl 0.1g, 1% (mas...

Embodiment 2

[0041] Embodiment 2 Green manure planting and overturning

[0042] The field experiment was carried out in the flue-cured tobacco base of Pingdi Town, Renhe District, Panzhihua City. The experiment was designed with 3 treatments, CK (open space in winter, no green manure planted), Vs (arrowhead pea green manure not inoculated with Rhizobium), Vs+R (inoculated with Rhizobium Arrowhead pea green manure, R is the rhizobium JSP1-3-1 bacterial agent prepared in Example 1).

[0043] Select the plump Arrowhead pea seeds in northern Sichuan, and mix the Arrowhead pea with the fungus agent. The sowing rate of the Arrowhead pea is 8.5kg / mu, and the amount of the bacteria agent is 1kg / mu. The seeds are sown on October 7, 2015. Before sowing Use the rotary tiller to plow the land, and then use the rotary tiller to turn the seeds on the soil surface into the soil. No chemical fertilizers are used during the growth of the arrowhead pea, and drainage ditches are properly dug to ensure draina...

Embodiment 3

[0051] Embodiment 3 flue-cured tobacco planting test effect

[0052] Tobacco was planted on the basis of Example 2, and the experimental design was 3 treatments, CF100% (Panzhihua Tobacco Company recommended fertilization rate, i.e. full fertilization), CF80%+Vs (the production of arrowhead pea is not inoculated with rhizobia, and tobacco is planted after pressing But only with 80% of the full amount of fertilization), CF80%+Vs+R (the production of arrowhead pea is inoculated with high-efficiency rhizobium JSP1-3-1, and the planting of tobacco after overturning only uses 80% of the full amount of fertilization). Each treatment was repeated 3 times and arranged in random blocks. Residential Area 24m 2 , 40 tobacco seedlings.

[0053] Table 3 The influence of arrowhead peas inoculated with Rhizobium on the yield and value of flue-cured tobacco

[0054]

[0055] It can be seen from Table 3 that the yield of CF80%+Vs treatment increased by 10.1% compared with CF100%, and the...

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Abstract

A fertilization-reducing and synergism-improving method of Vicia sativa L. inoculated with a rhizobium in tobacco land crop rotation comprises the following steps: 1, planting green manure: (1) carrying out amplification culture on a purified strain with peat and an assistant as a carrier until a logarithmic growth stage is reached, and fully and uniformly mixing the above formed bacterial suspension with sterilized peat under aseptic conditions to prepare a bacterial agent; and (2) performing seed dressing according to a mass ratio of the Vicia sativa L. to the bacterial agent of (7-10):1 under a field condition, drying the seeds in a shady place, performing broadcast sowing, overturning and pressing soil to make the seeds enter soil, and routinely irrigating the soil without applying a chemical fertilizer; 2, completely overturning and pressing Vicia sativa L. plants in a position 10-20 cm lower than the top of the soil when the Vicia sativa L. plants grow to a full-bloom stage; and3, carrying out seedling growth and irrigation in a routine manner during tobacco plantation, reducing the application amount of the chemical fertilizer by 20%, and carrying out harvesting and index determination after a mature stage is reached. The method has the advantages of reduction of the application amount of the chemical fertilizer, improvement of the yield of tobacco and the quality of flue-cured tobacco, and provision of a new green manure-flue-cured tobacco crop rotation manner.

Description

technical field [0001] The invention belongs to the field of microbe application, and in particular relates to a method for reducing fertilization and increasing efficiency in tobacco field rotation of arrowhead peas inoculated with rhizobia. Background technique [0002] As a special economic crop, tobacco must create a good soil environment for tobacco growth in order to achieve high-quality tobacco production. With the rapid development of flue-cured tobacco production in China, long-term large-scale application of chemical fertilizers and unreasonable crop rotations have led to problems such as decline in tobacco leaf yield and quality, soil compaction, soil fertility decline, and reduced organic matter content, which have become bottlenecks for the sustainable development of tobacco leaf production. In this context, exploring a cultivation measure that can increase the yield and quality of tobacco leaves, improve the soil, and realize the sustainable development of toba...

Claims

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Application Information

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IPC IPC(8): A01C21/00A01G7/06A01G22/40A01G22/45
CPCA01G7/06A01C21/005
Inventor 陈远学徐开未刘明廖金山蒋帆陈强张小平陈翠平
Owner SICHUAN AGRI UNIV
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