Method for extracting and preparing panax notoginseng saponins from fresh panax notoginseng
The technology of total saponins of Panax notoginseng and Panax notoginseng is applied in the field of extraction and preparation of active components of plants, and can solve the problems of high impurity content, easy inactivation of total saponins of Panax notoginseng, and low purity of total saponins of Panax notoginseng.
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Embodiment 1
[0031] Take fresh Panax notoginseng as the feed material, add 75% v / v ethanol homogenate which is 10 times the weight of fresh Panax notoginseng to the fresh Sanqi 75% v / v ethanol homogenate, after homogenization, let it stand for 24h, filter to obtain filtrate II, combine filtrate I and filtrate II, and filter with suction until the filtrate is clear to obtain filtrate III; put filtrate III at a temperature ≤ 60°C and 0.08Mpa Concentrate until there is no alcohol smell to obtain the concentrated solution I; add water 8 times the weight of the concentrated solution I to the concentrated solution I to dilute the upper D101 macroporous adsorption resin, add fresh Panax notoginseng with 8 times the weight of water (that is, add 8 times the weight of the feed) weight of water), washed with water, and then 75% v / v ethanol eluate of 8 times the weight of fresh Panax notoginseng to obtain the ethanol eluate, and the ethanol eluate was passed through the decolorizing resin and then con...
Embodiment 2
[0033] Take fresh Panax notoginseng as the feed material, add 75% v / v ethanol homogenate of 8 times the weight of fresh Panax notoginseng to the fresh Panax notoginseng, let it stand for 24 hours after homogenization, filter to obtain filtrate I, and add 6 times the weight of the filter residue to the filter residue 75% v / v ethanol homogenate, after homogenization, let it stand for 24h, filter to obtain filtrate II, combine filtrate I and filtrate II, and filter with suction until the filtrate is clear to obtain filtrate III; put filtrate III at a temperature ≤ 60°C and 0.08Mpa Concentrate until there is no alcohol smell to obtain the concentrated solution I; add the concentrated solution I to water 6 times the weight of the concentrated solution I to dilute the upper AB-8 macroporous adsorption resin, add water 6 times the weight of the feed, wash with water, and add 6 times the weight of the feed. Double the weight of 85% v / v ethanol to obtain the ethanol eluate, the ethanol ...
Embodiment 3
[0035]Take fresh Panax notoginseng as the feed material, add 75% v / v ethanol homogenate which is 8 times the weight of fresh Panax notoginseng to the fresh Panax notoginseng, let it stand for 12 hours after homogenization, filter to obtain filtrate I, add 5 times the weight of the filter residue to the filter residue 75% v / v ethanol homogenate, after homogenization, let it stand for 12h, filter to obtain filtrate II, combine filtrate I and filtrate II, and filter with suction until the filtrate is clear to obtain filtrate III; put filtrate III at a temperature ≤ 60°C and 0.08Mpa Concentrate until there is no alcohol smell to obtain the concentrated solution I; add the concentrated solution I to water 5 times the weight of the concentrated solution I to dilute the upper D101 macroporous adsorption resin, add water 5 times the weight of the feed, wash with water, and add 6 times the weight of the feed Wash with 80% v / v ethanol to obtain ethanol eluate, and then concentrate the et...
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