Novel lactic acid bacteria, composition containing lactic acid bacteria and application thereof

A composition and technology of Lactococcus, applied in the field of probiotics, can solve problems such as affecting the health of patients and being unable to remove wastes from the body

Active Publication Date: 2018-06-12
NEW BELLUS ENTERPRISES
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Patients with chronic kidney disease are often unable to remove waste from the body due to the loss of normal kidney function, allowing toxins such as uremic substances to enter the human body through the gastrointestinal tract, thereby affecting the health of patients. A method that can effectively delay or treat chronic kidney disease can be obtained from it

Method used

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  • Novel lactic acid bacteria, composition containing lactic acid bacteria and application thereof
  • Novel lactic acid bacteria, composition containing lactic acid bacteria and application thereof
  • Novel lactic acid bacteria, composition containing lactic acid bacteria and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0026] Embodiment one: Bacterial strain isolation and identification

[0027] Lactococcus subspecies LL358 and Lactobacillus salivarius LS159 were isolated from plant pickles, such as figure 1 and figure 2 shown.

[0028] According to the identification results of Taiwan Food Industry Development Institute, Lactococcus subspecies LL358 and Lactobacillus salivarius LS159 have the following characteristics: Gram-positive cocci, no catalyst, oxidase and motility, and no endospore production , can grow in both aerobic and anaerobic environments, and can be cultured in Lactobacillus MRS medium at a culture temperature of 37°C.

[0029] see again image 3, the 16S rDNA sequence of Lactococcus subspecies LL358 is shown in SEQ ID No.1. According to the 16SrDNA sequence analysis, it can be seen that Lactococcus lactis subsp. LL358 has a similarity of more than 98% with Lactococcus lactis subsp. Identification system identification confirmed that Lactococcus subsp. LL358 was close...

Embodiment 2

[0031] Embodiment two: prepare lactic acid bacteria composition

[0032] Lactococcus lactis subsp. Lactis LL358 CGMCC13317, Lactobacillus salivarius LS159 CGMCC 13316 and Lactobacillus pentosus were mixed at a ratio of 1:1:1 to prepare a lactic acid bacteria composition.

[0033] Wherein the Lactobacillus pentosus (Lactobacillus pentosus) was purchased from Food Industry Development Research Institute (BCRC), Hsinchu, Taiwan, and the number is: BCRC No.17973.

Embodiment 3

[0034] Embodiment three: Human experiment

[0035] To illustrate first, this example was approved by the "Member of Institutional Review Board, Tungs' Taichung Metro Harbor Hospital" (IRB No.TTMH: 102023).

[0036] A total of 80 patients with chronic kidney disease stage 3 and 4 were screened out by the Nephrology Clinic of Rentong General Hospital of Tongtong General Medical Association, and 29 patients withdrew from the trial due to personal reasons, and the remaining patients signed consent forms to become subjects By.

[0037] The subjects were randomly divided into a test group and a placebo group, wherein the test group administered a pack of 3 grams of lactic acid bacteria composition to each subject in the morning and evening, with a total bacterial count of 1×10 11 CFU / bag, the placebo group was given the same dose of resistant starch in the morning and evening. During the test period (0-12 weeks) and follow-up for 4 weeks (13-16 weeks) after fasting, each subject s...

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Abstract

The invention discloses novel lactic acid bacteria. The novel lactic acid bacteria comprise separated lactic acid bacteria subspecies LL358, which is deposited in Chinese Academy of Sciences Instituteof Microbiology, with the deposition number of CGMCC 13317 and the deposition date of Nov. 18th, 2016 and separated saliva lactic acid bacteria LS159, which is deposited in Chinese Academy of Sciences Institute of Microbiology, with the deposition number of CGMCC 13316 and the deposition date of Nov. 18th, 2016. Through the novel lactic acid bacteria disclosed by the invention, the diseases suchas renal injury, metabolic syndrome, hyperuricemia and hypocalcemia can be improved or cured effectively.

Description

technical field [0001] The present invention relates to probiotics and uses thereof, in particular to a novel lactic acid bacterium, a composition containing the lactic acid bacterium and uses thereof. Background technique [0002] According to the Taiwan Renal Registry Data System (TWRDS) database from 2005 to 2012, the incidence of end stage renal disease (ESRD) in Taiwan has increased from 376 per million people to 426 people. According to the statistics of Taiwan Ministry of Health and Welfare in 2014, among the top ten causes of death in 2013, nephritis, nephrotic syndrome and nephropathy ranked tenth, accounting for 2.9% of the total number of deaths. The proportion of adults over 20 years old in China suffering from Chronic Kidney Disease (CKD) is 10%. Chronic kidney disease can be divided into 5 stages according to the estimated glomerular filtration rate (eGFR) and whether there is substantial damage to the kidneys. Among them, stage 1-2 (eGFR>60mL / min / 1.73m 2...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20A61K35/747A61P13/12A61P3/00A61P19/06A61P3/02A23L33/135C12R1/01C12R1/225A61K35/744
CPCA61K35/744A61K35/747C12N1/20C12N1/205C12R2001/225C12R2001/46A61K2300/00
Inventor 黄俊智黄文颖王雪芳林柏松刘品秀
Owner NEW BELLUS ENTERPRISES
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