Recombinase polymerase isothermal amplification and test strip combined method for detection of Staphylococcus aureus and application thereof

Abstract

The invention discloses a recombinase polymerase isothermal amplification and test strip combined method for detection of Staphylococcus aureus. The method includes the steps of: designing a specificStaphylococcus aureus primer pair, taking the Staphylococcus aureus genomic DNA as the template, conducting recombinase polymerase isothermal amplification, and applying a colloidal gold test strip toacquire the detection result. The method is rapid and simple, has no need for expensive instruments and equipment, can generate a detectable level product only through 10min amplification under a 40DEG C constant temperature condition, and does not need a tedious electrophoresis process, a result can be acquired within 5min of colloidal gold test strip detection, and the result can be observed bynaked eyes. In addition, the method has strong specificity, is free of reaction with other bacterial genuses, has high sensitivity, and makes up for the disadvantages of the existing traditional detection technology. The method is expected to be applied to grass-roots inspection and quarantine institutions and become a conventional detection means, and is of important significance for improving food hygiene level, ensuring food safety and promoting the international food trade development.

Description

technical field [0001] The invention belongs to the technical field of microbial detection, and relates to a rapid detection technology for constant temperature amplification of Staphylococcus aureus, in particular to a method and application of a recombinant enzyme polymerase constant temperature amplification combined with a test strip to detect Staphylococcus aureus. Background technique [0002] Staphylococcus aureus (Staphylococcus aureus) widely exists in nature, mainly polluting meat, dairy products and vegetables, etc., can cause human and animal diseases, and can lead to various types of infections, such as: skin and soft tissue infection, arthritis , cellulitis, mastitis, necrotizing pneumonia, orbital cellulitis, endocarditis and sepsis. Staphylococcus aureus is one of the most important foodborne pathogenic microorganisms causing food poisoning worldwide. The U.S. Centers for Disease Control (CDC) report that 33% of all bacterial food poisoning incidents are cau...

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Problems solved by technology

[0005] Through the search, no patent publications related to the application of the present invention have been found

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