STED (stimulated emission depletion) parallel microscopic imaging system based on uniform structured illumination

Abstract

The invention provides an STED (stimulated emission depletion) parallel microscopic imaging system based on uniform structured illumination. By designing an illumination module, an exciting light beamemitted by an exciting light laser is divided into two coherent light beams, a depletion light beam of Gaussian distribution is modulated into a flattened beam by a flattened Gaussian beam shaper andis divided into two coherent light beams, uniformly distributed streaky exciting structured light and depletion structured light are obtained respectively by interference, excitation and depletion are performed on a sample, STED parallel microscopic imaging is performed, then image reconstruction is performed with an STED coordinate positioning method and an SIM frequency domain and spectrogram fusion method, super-resolution imaging is realized, and the field range and the imaging speed of the STED microscopic system can be increased.

Description

technical field [0001] The invention relates to the field of design and manufacture of microscopic detection instruments, in particular to a STED parallel microscopic imaging system based on uniform structured light illumination. Background technique [0002] Super-resolution microscopic imaging technology is a cutting-edge technology used in biological and other research fields. It is mainly divided into two types of microscopic imaging methods: coordinate positioning and coordinate random. Among them, the typical representative of the coordinate positioning microscopic imaging method is stimulated radiation depletion (STED) microscopic imaging. STED microscopic imaging directly uses optical methods to scan the sample point by point to achieve super-resolution imaging. However, limited by the single-point scanning method, the imaging speed of STED microscopic imaging is relatively slow. [0003] In recent years, in order to improve the imaging speed of STED microscopic ima...

AI Technical Summary

Problems solved by technology

However, due to the addition of two-dimensional structured light, the complexity of the imaging system becomes higher, and the excitation light in this technology does not use structured light mode but uses wide-field excitation, which significantly reduces the signal-to-noise ratio of effective fluorescence and affects the imaging resolution.

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