Preparation method of atherosclerotic mouse model with NOD (Non-Obese Diabetic) genetic background

An atherosclerosis, mouse model technology, applied in the field of genetic engineering and genetic modification, can solve the problem of lack of NOD genetic background mouse gene knockout model, and achieve the effect of saving reagents and time cost, and high shearing efficiency

Active Publication Date: 2018-07-06
XINXIANG MEDICAL UNIV
View PDF2 Cites 3 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

NOD mice are a non-obese diabetic strain, and the cumulative incidence of diabetes at the age of 30 weeks is 60-80% for males and 20-30% for males, respectively. Female mice have insulin-dependent diabetes, and the clinical symptoms are quite similar to human type I

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Preparation method of atherosclerotic mouse model with NOD (Non-Obese Diabetic) genetic background
  • Preparation method of atherosclerotic mouse model with NOD (Non-Obese Diabetic) genetic background
  • Preparation method of atherosclerotic mouse model with NOD (Non-Obese Diabetic) genetic background

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0052] The preparation method of the atherosclerosis mouse model of NOD genetic background in this embodiment (flow process is as follows figure 1 shown), including the following steps:

[0053] 1. According to the principle of CRISPR / Cas9 system, use CRISPOR online design software to determine the specific target sites of ApoE (Gene ID: 88057) and LDLR (Gene ID: 96765) in NOD mice sgRNA2, ApoE-sgRNA3 and LDLR-sgRNA1, LDLR-sgRNA2, LDLR-sgRNA3.

[0054] Find the mouse ApoE (Transcript ID: ENSMUST00000174064.8) and LDLR (Transcript ID: ENSMUST00000034713.8) gene DNA sequences in the mouse genome database ensembl (http: / / asia.ensembl.org), and then use the online design software CRISPOR (http: / / crispor.tefor.net / crispor.cgi), determined to select 3 specific sites in exon3 (exon ID: ENSMUSE00000231320) of the mouse ApoE gene and exon4 (exon ID: ENSMUSE00000217581) of the LDLR gene As the target sequence of sgRNA, the three target sequences are:

[0055] ApoE-sgRNA1: CCTAGCCGAGG...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

No PUM Login to view more

Abstract

The invention relates to a preparation method of an atherosclerotic mouse model with NOD (Non-Obese Diabetic) genetic background, and belongs to the technical fields of genetic engineering and geneticmodification. For the first time, ApoE (Apolipoprotein E) and LDLR (Low-Density Lipoprotein Receptor) genes in a NOD genetic background mouse are knocked out at the same time to obtain a gene knockout mouse model, which can induce atherosclerotic atherosclerosis, of the NOD genetic background mouse. The aorta of the atherosclerotic mouse model with the NOD genetic background has obvious atherosclerotic plaque formation. Compared with the traditional classic atherosclerotic model C57B/L6 background ApoE gene knockout mouse, the atherosclerotic mouse model has consistent incidence degree. By adopting the method disclosed by the invention, a brand new atherosclerosis gene knockout mouse model with the NOD genetic background is provided for researchers, and is of great value for the basic theoretical research as well as clinical diagnosis and treatment of atherosclerosis.

Description

technical field [0001] The invention relates to a method for preparing an atherosclerosis mouse model with NOD genetic background, belonging to the technical field of genetic engineering and genetic modification. Background technique [0002] Atherosclerosis is one of the main causes of cardiovascular and cerebrovascular diseases. In-depth research on its mechanism will help to solve the difficult problem of curing and high mortality of cardiovascular and cerebrovascular diseases. NOD mice are a non-obese diabetic strain, and the cumulative incidence of diabetes at the age of 30 weeks is 60-80% for males and 20-30% for males, respectively. Female mice have insulin-dependent diabetes, and the clinical symptoms are quite similar to human type I diabetes. Mice are the most widely used mouse strains in the research of type 1 diabetes and the establishment of humanized models, but gene knockout models of NOD genetic background mice are still extremely scarce, especially in the fi...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
IPC IPC(8): C12N15/90A01K67/027
CPCA01K67/0276A01K2217/075A01K2227/105A01K2267/0375C12N15/907C12N2800/80C12N2810/10
Inventor 梁银明张黎琛卢燎勋王旭刚黄蓉晁天柱郑前前罗静谷妍蓉
Owner XINXIANG MEDICAL UNIV
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap