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Application of ENSG00000267549 in judgment of metastasis of osteosarcoma

A technology for osteosarcoma and products, applied in the field of biomedicine, can solve problems such as no specific model

Active Publication Date: 2018-07-06
杨祚璋
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Cytogenetic studies have shown that the development of osteosarcoma involves a variety of complex changes in some chromosomes, but without any specific pattern

Method used

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  • Application of ENSG00000267549 in judgment of metastasis of osteosarcoma
  • Application of ENSG00000267549 in judgment of metastasis of osteosarcoma

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0041] Example 1 Screening of lncRNAs associated with osteosarcoma

[0042] 1. Sample collection and clinical information

[0043] The tumor tissues of 3 patients with osteosarcoma metastases (metastatic lesions, the metastatic site is the lung) and 3 patients with non-metastatic osteosarcoma (primary lesions) were collected. The clinical information of the patients is shown in Table 1. The collection of all samples was approved by the ethics committee, and the patients gave informed consent.

[0044] Table 1 Clinical information of patients

[0045]

[0046] 2. Preparation of RNA samples

[0047] RNA samples were extracted using the tissue RNA extraction kit from Invitrogen, see the instruction manual for details.

[0048] 3. Quality analysis of RNA samples

[0049] Nanodrop2000 was used to detect the concentration and purity of the extracted RNA, agarose gel electrophoresis was used to detect RNA integrity, and Agilent2100 was used to determine the RIN value. Concent...

Embodiment 2

[0056] Example 2 QPCR verification of differentially expressed ENSG00000267549

[0057] 1. According to the high-throughput sequencing results, ENSG00000267549 was selected for large-sample QPCR verification. According to the sample collection method in Example 1, 36 patients with primary tumor and 33 patients with tumor metastasis were selected.

[0058] 2. RNA extraction

[0059] RNA samples were extracted using the tissue RNA extraction kit from Invitrogen, see the instruction manual for details.

[0060] 3. Reverse transcription:

[0061] 1) Mix 10pg-1μg total RNA template with 2μl 10× buffer, 2μl dATP (10mM), 0.5μl polyA polymerase, 0.5μl RNase inhibitor and RNase free water , the final volume was 20 μl, and incubated at 37°C for 1h.

[0062] 2) Add 1 μl of 0.5 μg / μl Oligo(dT) specific RT primer to the reaction tube, and incubate at 70° C. for 5 minutes.

[0063] 3) Immediately incubate on ice for at least 2 minutes to break the secondary structure of RNA and primers...

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Abstract

The invention discloses application of ENSG00000267549 in judgment of metastasis of osteosarcoma. High-throughput sequencing finds that the ENSG00000267549 is obviously increased in a patient suffering from metastasis of osteosarcoma; and further research finds that the ENSG00000267549 is related to metastasis of osteosarcoma. Whether the metastasis of osteosarcoma occurs or not can be judged by detecting the expression level of the ENSG00000267549.

Description

technical field [0001] The invention belongs to the field of biomedicine and relates to the application of ENSG00000267549 in judging the metastasis of osteosarcoma. Background technique [0002] Osteosarcoma (OS) is a common malignant bone-forming tumor, the most common primary malignant tumor in clinical diagnosis, and the most common patients are children and adolescents. The tumor is an aggressive malignancy of mesenchymal-derived primitive transformed cells exhibiting osteoblastic differentiation, producing malignant osteoid. The tumor body of osteosarcoma is often spindle-shaped, and the pathological cut surface of the tumor body is brown-red or off-white fish-like, and the cortex, medulla, and periosteum of the bone may all be affected parts. Osteosarcoma is classified according to tissue type and can be divided into osteoblastic osteosarcoma, chondroblastic osteosarcoma and fibroblastic osteosarcoma. [0003] Typical osteosarcoma is rare in clinical cases, only 3 p...

Claims

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Application Information

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IPC IPC(8): C12Q1/6886
CPCC12Q1/6886C12Q2600/124C12Q2600/156
Inventor 杨祚璋何泽伟黄泽勇杨义豪张娅李素
Owner 杨祚璋