Ophthalmic composition
A technology of composition and application, applied in the direction of glasses/protective glasses, glasses/goggles, disinfection, etc., can solve problems such as inaccessibility, moisture locking, corneal toxicity damage, etc.
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[0028] Example 1. Cytotoxicity and Antibacterial Properties of Ophthalmic Compositions
[0029] Materials and methods
[0030] 1. Ophthalmic composition formula
[0031] The formulation of the ophthalmic composition used in this example is shown in Table 1 below.
[0032] Table 1. Formulation (100ml) of the ophthalmic composition
[0033]
[0034] Also contains buffer components: CaCl 2 0.015g, KCl 0.15g, NaCl 0.45g, NaCl 2 HPO 4 0.45-1.8g, pH=6.5-7.5, and surfactant Poloxamer-407, 0.05-0.5g.
[0035] 2. Cytotoxicity test
[0036] Take 100 μL, the concentration is 1×10 5 Cells / mL of L929 (mouse lung fibroblast cell line, Food Industry Development Research Institute of the Foundation, registration number BCRC 60091) cell suspension was added to a 96-well plate, and placed in an environment containing 5±1% carbon dioxide and a temperature of 37±1°C Incubate for 24±2 hours. Aspirate the culture solution, add 100 μL of the prepared ophthalmic composition dilution (ad...
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